Regulation of MAP4K4 gene expression by RNA interference through an engineered theophylline-dependent hepatitis delta virus ribozyme switch
文献类型：期刊论文
| 作者 | Cheng, H; Zhang, YY; Wang, HY; Sun, N; Liu, M; Chen, HX; Pei, RJ(裴仁军) |
| 刊名 | MOLECULAR BIOSYSTEMS
 |
| 出版日期 | 2016 |
| 卷号 | 12期号:11 |
| 通讯作者 | Pei, RJ(裴仁军) |
| 英文摘要 | Riboswitches are functional non-coding RNA regulatory components that play an important role in the regulation of gene expression in diverse organisms. In particular, using riboswitches to modulate RNA interference (RNAi) enables temporal and spatial control of gene expression in mammalian cells. Herein, a ribozyme gene switch to activate RNAi was fabricated for the artificial regulation of versatile gene silencing through the interaction of an RNA aptamer with small molecules. The device comprised an allosteric HDV ribozyme with an embedded theophylline aptamer and a primary miRNA (pri-miRNA) to silence the MAP4K4 gene in hepatic (HepG2) cells, aiming to achieve dose-dependent control of the activation of RNAi, and then the regulation of the MAP4K4 gene by theophylline. Finally, we demonstrated the feasibility and applicability of utilizing HDV ribozyme switches to activate RNAi for regulating an endogenous gene in mammalian cells. |
| 关键词[WOS] | MAMMALIAN-CELLS ; REQUIREMENTS ; RIBOSWITCHES |
| 收录类别 | SCI ; EI |
| 语种 | 英语 |
| WOS记录号 | WOS:000386669400014 |
| 源URL | [http://ir.sinano.ac.cn/handle/332007/4905]  |
| 专题 | 苏州纳米技术与纳米仿生研究所_纳米生物医学与安全研究部_裴仁军团队 |
| 推荐引用方式 GB/T 7714 | Cheng, H,Zhang, YY,Wang, HY,et al. Regulation of MAP4K4 gene expression by RNA interference through an engineered theophylline-dependent hepatitis delta virus ribozyme switch[J]. MOLECULAR BIOSYSTEMS,2016,12(11). |
| APA | Cheng, H.,Zhang, YY.,Wang, HY.,Sun, N.,Liu, M.,...&Pei, RJ.(2016).Regulation of MAP4K4 gene expression by RNA interference through an engineered theophylline-dependent hepatitis delta virus ribozyme switch.MOLECULAR BIOSYSTEMS,12(11). |
| MLA | Cheng, H,et al."Regulation of MAP4K4 gene expression by RNA interference through an engineered theophylline-dependent hepatitis delta virus ribozyme switch".MOLECULAR BIOSYSTEMS 12.11(2016). |
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