Magnetic Resonance Imaging Revealed Splenic Targeting of Canine Parvovirus Capsid Protein VP2
文献类型：期刊论文
| 作者 | Ma, YF; Wang, HM; Yan, D; Wei, YQ; Cao, YH(曹玉华); Yi, PW(易佩伟); Zhang, HL(张海禄); Deng, ZW(邓宗武); Dai, JW(戴建武); Liu, XT |
| 刊名 | SCIENTIFIC REPORTS
 |
| 出版日期 | 2016 |
| 卷号 | 6 |
| 通讯作者 | Sun, SQ ; Guo, HC ; Zhang, ZJ(张智军) |
| 英文摘要 | Canine parvovirus (CPV) is a highly contagious infectious virus, whose infectious mechanism remains unclear because of acute gastroenteritis and the lack of an efficient tool to visualize the virus in real time during virology research. In this study, we developed an iron oxide nanoparticle supported by graphene quantum dots (GQD), namely, FeGQD. In this composite material, GQD acts as a stabilizer; thus, vacancies are retained on the surface for further physical adsorption of the CPV VP2 protein. The FeGQD@VP2 nanocomposite product showed largely enhanced colloidal stability in comparison with bare FeGQD, as well as negligible toxicity both in vitro and in vivo. The composite displayed high uptake into transferrin receptor (TfR) positive cells, which are distinguishable from FeGQD or TfR negative cells. In addition, the composite developed a significant accumulation in spleen rather than in liver, where bare FeGQD or most iron oxide nanoparticles gather. As these evident targeting abilities of FeGQD@VP2 strongly suggested, the biological activity of CPV VP2 was retained in our study, and its biological functions might correspond to CPV when the rare splenic targeting ability is considered. This approach can be applied to numerous other biomedical studies that require a simple yet efficient approach to track proteins in vivo while retaining biological function and may facilitate virus-related research. |
| 关键词[WOS] | VIRUS-LIKE PARTICLES ; NANOPARTICLES ; BIODISTRIBUTION ; TOXICITY ; CLEARANCE ; VARIANTS ; MICE ; DOGS |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000372512500001 |
| 源URL | [http://ir.sinano.ac.cn/handle/332007/4750]  |
| 专题 | 苏州纳米技术与纳米仿生研究所_纳米生物医学与安全研究部_张智军团队 |
| 推荐引用方式 GB/T 7714 | Ma, YF,Wang, HM,Yan, D,et al. Magnetic Resonance Imaging Revealed Splenic Targeting of Canine Parvovirus Capsid Protein VP2[J]. SCIENTIFIC REPORTS,2016,6. |
| APA | Ma, YF.,Wang, HM.,Yan, D.,Wei, YQ.,Cao, YH.,...&Guo, HC.(2016).Magnetic Resonance Imaging Revealed Splenic Targeting of Canine Parvovirus Capsid Protein VP2.SCIENTIFIC REPORTS,6. |
| MLA | Ma, YF,et al."Magnetic Resonance Imaging Revealed Splenic Targeting of Canine Parvovirus Capsid Protein VP2".SCIENTIFIC REPORTS 6(2016). |
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