中国科学院机构知识库网格
Chinese Academy of Sciences Institutional Repositories Grid
An electrochemical aptasensor electrocatalyst for detection of thrombin

文献类型:期刊论文

作者Tian, R; Chen, XJ; Li, QW(李清文); Yao, C
刊名ANALYTICAL BIOCHEMISTRY
出版日期2016
卷号500
通讯作者Tian, R ; Yao, C
英文摘要This work reports a novel signal amplification strategy based on three-dimensional ordered macroporous C-60-poly(3,4-ethylenedioxythiophene)-1-butyl-3-methylimidazolium hexafluorophosphate (3DOM C-60-PEDOT-[BMIm][BF6]) for ultrasensitive detection of thrombin by cascade catalysis of Au-PEDOT@SiO2 microspheres and alcohol dehydrogenase (ADH). Au-PEDOT@SiO2 microspheres were constructed not only as nanocarriers to anchor the large amounts of secondary thrombin aptamers but also as nano catalysts to catalyze the oxidation of ethanol efficiently. Significantly, the electrochemical signal was greatly enhanced based on cascade catalysis: First, ADH catalyzed the oxidation of ethanol to acetaldehyde with the concomitant generation of NADH in the presence of beta-nicotinamide adenine dinucleotide hydrate (NADI(+)). Then, gold nanoparticles (AuNPs) as nanocatalysts could effectively catalyze NADH to produce NAD(+) with the help of PEDOT as redox probe. Under the optimal conditions, the proposed aptasensor exhibits a linear range of 2 x 10(-13) to 2 x 10(-8) M with a low detection limit of 2 x 10(-14) M for thrombin detection and shows high sensitivity and good specificity. (C) 2016 Elsevier Inc. All rights reserved.
关键词[WOS]HIGHLY SENSITIVE DETECTION ; GOLD NANOPARTICLES ; CARBON NANOTUBE ; AMPLIFICATION APTASENSOR ; DUAL-AMPLIFICATION ; SOLAR-CELLS ; ELECTRODE ; MICROSPHERES ; POLYANILINE ; QUADRUPLEX
收录类别SCI
语种英语
WOS记录号WOS:000373968700015
源URL[http://ir.sinano.ac.cn/handle/332007/4699]  
专题苏州纳米技术与纳米仿生研究所_先进材料研究部_李清文团队
推荐引用方式
GB/T 7714
Tian, R,Chen, XJ,Li, QW,et al. An electrochemical aptasensor electrocatalyst for detection of thrombin[J]. ANALYTICAL BIOCHEMISTRY,2016,500.
APA Tian, R,Chen, XJ,Li, QW,&Yao, C.(2016).An electrochemical aptasensor electrocatalyst for detection of thrombin.ANALYTICAL BIOCHEMISTRY,500.
MLA Tian, R,et al."An electrochemical aptasensor electrocatalyst for detection of thrombin".ANALYTICAL BIOCHEMISTRY 500(2016).

入库方式: OAI收割

来源:苏州纳米技术与纳米仿生研究所

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