Application of RAD Sequencing for Evaluating the Genetic Diversity of Domesticated Panax notoginseng (Araliaceae)
文献类型：期刊论文
| 作者 | Pan, Yuezhi1,2 ; Wang, Xueqin3; Sun, Guiling4; Lie, Fusheng2; Gong, Xun1
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| 刊名 | PLOS ONE
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| 出版日期 | 2016-11-15 |
| 卷号 | 11期号:11页码:166419 |
| 英文摘要 | Panax notoginseng, a traditional Chinese medicinal plant, has been cultivated and domesticated for approximately 400 years, mainly in Yunnan and Guangxi, two provinces in southwest China. This species was named according to cultivated rather than wild individuals, and no wild populations had been found until now. The genetic resources available on farms are important for both breeding practices and resource conservation. In the present study, the recently developed technology RADseq, which is based on next-generation sequencing, was used to analyze the genetic variation and differentiation of P. notoginseng. The nucleotide diversity and heterozygosity results indicated that P. notoginseng had low genetic diversity at both the species and population levels. Almost no genetic differentiation has been detected, and all populations were genetically similar due to strong gene flow and insufficient splitting time. Although the genetic diversity of P. notoginseng was low at both species and population levels, several traditional plantations had relatively high genetic diversity, as revealed by the He and p values and by the private allele numbers. These valuable genetic resources should be protected as soon as possible to facilitate future breeding projects. The possible geographical origin of Sanqi domestication was discussed based on the results of the genetic diversity analysis. |
| 类目[WOS] | Multidisciplinary Sciences |
| 研究领域[WOS] | Science & Technology - Other Topics |
| 关键词[WOS] | MULTILOCUS GENOTYPE DATA ; POPULATION-STRUCTURE ; NATURAL-POPULATIONS ; DE-NOVO ; MOLECULAR EVOLUTIONARY ; SNP DISCOVERY ; HISTORY ; DNA ; MARKERS ; PLANTS |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000387794600061 |
| 源URL | [http://ir.kib.ac.cn/handle/151853/33784]  |
| 专题 | 昆明植物研究所_中国科学院东亚植物多样性与生物地理学重点实验室 |
| 作者单位 | 1.Chinese Acad Sci, Kunming Inst Bot, Key Lab Plant Div & Biogeog East Asia, Kunming, Peoples R China 2.Yunnan Agr Univ, Coll Agron & Biotechnol, Kunming, Peoples R China 3.Zhoukou Normal Univ, Coll Life Sci & Agron, Zhoukou, Peoples R China 4.Yunnan Key Lab Wild Plant Resources, Kunming, Peoples R China |
| 推荐引用方式 GB/T 7714 | Pan, Yuezhi,Wang, Xueqin,Sun, Guiling,et al. Application of RAD Sequencing for Evaluating the Genetic Diversity of Domesticated Panax notoginseng (Araliaceae)[J]. PLOS ONE,2016,11(11):166419. |
| APA | Pan, Yuezhi,Wang, Xueqin,Sun, Guiling,Lie, Fusheng,&Gong, Xun.(2016).Application of RAD Sequencing for Evaluating the Genetic Diversity of Domesticated Panax notoginseng (Araliaceae).PLOS ONE,11(11),166419. |
| MLA | Pan, Yuezhi,et al."Application of RAD Sequencing for Evaluating the Genetic Diversity of Domesticated Panax notoginseng (Araliaceae)".PLOS ONE 11.11(2016):166419. |
入库方式： OAI收割
来源：昆明植物研究所
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