Overexpression of glucose-6-phosphate dehydrogenase enhanced the polyunsaturated fatty acid composition of Aurantiochytrium sp SD116
文献类型：期刊论文
| 作者 | Cui, Gu-Zhen1; Ma, Zengxin1,2; Liu, Ya-Jun1; Feng, Yingang1; Sun, Zhijie1; Cheng, Yurong1,2; Song, Xiaojin1; Cui, Qiu1 |
| 刊名 | ALGAL RESEARCH-BIOMASS BIOFUELS AND BIOPRODUCTS
 |
| 出版日期 | 2016-11-01 |
| 卷号 | 19页码:138-145 |
| 关键词 | Aurantiochytrium Polyunsaturated fatty acid Glucose-6-phosphate dehydrogenase NADPH |
| 英文摘要 | Aurantiochytrium sp. (Thraustochytriaceae, Stramenopiles) is a highly promising polyunsaturated fatty acid (PUFA) resource due to its high docosahexaenoic acid (DHA) production. The reducing agent NADPH is essential for the production of PUFA and other fatty acids, and glucose-6-phosphate dehydrogenase (G6PDH) is one of the most important enzymes for NADPH regeneration in Aurantiochytrium. To investigate whether PUFA production could be enhanced by stimulating G6PDH, a recombinant strain, termed SD116::G6PDH, was constructed by overexpressing G6PDH in Aurantiochytriumsp. SD116. Although decreased cell growth and fatty acid production were observed, G6PDH overexpression changed the fatty acid profile and enhanced the proportion of PUFA in lipids by 10.6%. These results confirmed the positive effect of G6PDH activity on PUFA production and suggested that the manipulation of NADPH production is a promising strategy for increasing PUFA production in Aurantiochytrium. (C) 2016 Elsevier B.V. All rights reserved. |
| WOS标题词 | Science & Technology ; Life Sciences & Biomedicine |
| 类目[WOS] | Biotechnology & Applied Microbiology |
| 研究领域[WOS] | Biotechnology & Applied Microbiology |
| 关键词[WOS] | GENE PHYLOGENY THRAUSTOCHYTRIACEAE ; DOCOSAHEXAENOIC ACID ; MALIC ENZYME ; MICROALGA SCHIZOCHYTRIUM ; TAXONOMIC REARRANGEMENT ; TRANSGENE EXPRESSION ; MUCOR-CIRCINELLOIDES ; LIPID-METABOLISM ; SYSTEM ; DHA |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000390001400017 |
| 源URL | [http://ir.qibebt.ac.cn/handle/337004/9205]  |
| 专题 | 青岛生物能源与过程研究所_代谢物组学团队 |
| 作者单位 | 1.Chinese Acad Sci, Qingdao Inst Bioenergy & Bioproc Technol, Qingdao Engn Lab Single Cell Oil, Shandong Prov Key Lab Energy Genet,Key Lab Biofue, Qingdao, Shandong, Peoples R China 2.Univ Chinese Acad Sci, Beijing, Peoples R China |
| 推荐引用方式 GB/T 7714 | Cui, Gu-Zhen,Ma, Zengxin,Liu, Ya-Jun,et al. Overexpression of glucose-6-phosphate dehydrogenase enhanced the polyunsaturated fatty acid composition of Aurantiochytrium sp SD116[J]. ALGAL RESEARCH-BIOMASS BIOFUELS AND BIOPRODUCTS,2016,19:138-145. |
| APA | Cui, Gu-Zhen.,Ma, Zengxin.,Liu, Ya-Jun.,Feng, Yingang.,Sun, Zhijie.,...&Cui, Qiu.(2016).Overexpression of glucose-6-phosphate dehydrogenase enhanced the polyunsaturated fatty acid composition of Aurantiochytrium sp SD116.ALGAL RESEARCH-BIOMASS BIOFUELS AND BIOPRODUCTS,19,138-145. |
| MLA | Cui, Gu-Zhen,et al."Overexpression of glucose-6-phosphate dehydrogenase enhanced the polyunsaturated fatty acid composition of Aurantiochytrium sp SD116".ALGAL RESEARCH-BIOMASS BIOFUELS AND BIOPRODUCTS 19(2016):138-145. |
入库方式： OAI收割
来源：青岛生物能源与过程研究所
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