Quinary Interactions Weaken the Electric Field Generated by Protein Side-Chain Charges in the Cell-like Environment
文献类型：期刊论文
| 作者 | Zhang, Ning1,2,3; An, Liaoyuan1,2 ; Li, Jingwen1,2,3; Liu, Zhijun4; Yao, Lishan1,2
|
| 刊名 | JOURNAL OF THE AMERICAN CHEMICAL SOCIETY
 |
| 出版日期 | 2017-01-18 |
| 卷号 | 139期号:2页码:647-654 |
| 英文摘要 | The intramolecular electric field (e-field) generated by protein GB3 side-chain charges K/E10, K/E19, and D/K40 was measured in the absence or presence of macromolecular crowding. The e-field responds differently to different crowding agents-dextran, Ficoll, BSA, and E. coli cell lysate. Dextran and Ficoll have no effect on the e-field. The lysate generally weakens the e-field but the amplitude of weakening varies greatly. For example, the e-field by K19 is reduced by 67% in the presence of 90 g/L lysate, corresponding to a charge change from 0.9 to 0.3 e for K19, whereas the e-fields by D/K40 are weakened only by, similar to 7% under the same lysate concentration. The extent of the e-field weakening by BSA is in between that by Ficoll (dextran) and lysate. Further investigations, suggest that the e-field weakening mechanism by lysate is similar to that by NaCl. That is, the e-field generated by a protein surface charge affects the distribution of lysate which creates a reaction field and weakens the protein e-field. Our study indicates that the protein electrostatic property can be changed significantly due to quinary interaction with the cell environment. |
| WOS标题词 | Science & Technology ; Physical Sciences |
| 类目[WOS] | Chemistry, Multidisciplinary |
| 研究领域[WOS] | Chemistry |
| 关键词[WOS] | RESIDUAL DIPOLAR COUPLINGS ; NUCLEAR-MAGNETIC-RESONANCE ; NMR-SPECTROSCOPY ; LIVING CELLS ; CONFORMATIONAL EXCHANGE ; ESCHERICHIA-COLI ; LIQUID-CRYSTAL ; STABILITY ; DYNAMICS ; ANISOTROPY |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000392459300021 |
| 源URL | [http://ir.qibebt.ac.cn/handle/337004/9167]  |
| 专题 | 青岛生物能源与过程研究所_仿真模拟团队 |
| 作者单位 | 1.Chinese Acad Sci, Qingdao Inst Bioenergy & Bioproc Technol, Shandong Prov Key Lab Synthet Biol, Qingdao 266061, Peoples R China 2.Chinese Acad Sci, Qingdao Inst Bioenergy & Bioproc Technol, Lab Biofuels, Qingdao 266061, Peoples R China 3.Univ Chinese Acad Sci, Beijing 100049, Peoples R China 4.Chinese Acad Sci, Shanghai Inst Biol Sci, Inst Biochem & Cell Biol, Natl Ctr Prot Sci Shanghai, Shanghai 201210, Peoples R China |
| 推荐引用方式 GB/T 7714 | Zhang, Ning,An, Liaoyuan,Li, Jingwen,et al. Quinary Interactions Weaken the Electric Field Generated by Protein Side-Chain Charges in the Cell-like Environment[J]. JOURNAL OF THE AMERICAN CHEMICAL SOCIETY,2017,139(2):647-654. |
| APA | Zhang, Ning,An, Liaoyuan,Li, Jingwen,Liu, Zhijun,&Yao, Lishan.(2017).Quinary Interactions Weaken the Electric Field Generated by Protein Side-Chain Charges in the Cell-like Environment.JOURNAL OF THE AMERICAN CHEMICAL SOCIETY,139(2),647-654. |
| MLA | Zhang, Ning,et al."Quinary Interactions Weaken the Electric Field Generated by Protein Side-Chain Charges in the Cell-like Environment".JOURNAL OF THE AMERICAN CHEMICAL SOCIETY 139.2(2017):647-654. |
入库方式： OAI收割
来源：青岛生物能源与过程研究所
浏览0
下载0
收藏0
其他版本
除非特别说明，本系统中所有内容都受版权保护，并保留所有权利。