Immobilization of proline-specific endoprotease on nonporous silica nanoparticles functionalized with amino group
文献类型：期刊论文
| 作者 | Zhao, Fuhua1,2; Hou, Tonggang2; Wang, Jianxun2; Jiang, Yijun2; Huang, Shuxia1; Wang, Qiao2; Xian, Mo2; Mu, Xindong2 |
| 刊名 | BIOPROCESS AND BIOSYSTEMS ENGINEERING
 |
| 出版日期 | 2017 |
| 卷号 | 40期号:1页码:1-7 |
| 关键词 | Enzyme immobilization Proline-specific endoprotease Enzymatic activity Beer Chill-haze |
| 英文摘要 | Enzyme immobilization is believed to provide an excellent base for increasing environmental tolerance of enzyme and considerable period of time. In this work, a kind of nonporous silica nanoparticles functionalized with amino group was synthesized to immobilize proline-specific endoprotease (PSEP). PSEP is known to specifically cleave peptides (or esters) at the carboxyl side of proline, thus can prevent the formation of haze and prolong the shelf life of beer. After immobilization, the environmental tolerance (temperature and pH, respectively) was obviously improved, and the immobilized enzyme can retain above 90 % of its original activity after 6 uses. Moreover, the immobilized enzyme can effectively prevent the formation of chill-haze using fresh beer fermentation liquid. |
| WOS标题词 | Science & Technology ; Life Sciences & Biomedicine ; Technology |
| 类目[WOS] | Biotechnology & Applied Microbiology ; Engineering, Chemical |
| 研究领域[WOS] | Biotechnology & Applied Microbiology ; Engineering |
| 关键词[WOS] | ENZYME IMMOBILIZATION ; BEER POLYPEPTIDES ; HAZE FORMATION ; STABILIZATION ; POLYPHENOLS ; PROTEINS ; SYSTEM ; ACID ; GEL |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000391382800001 |
| 源URL | [http://ir.qibebt.ac.cn/handle/337004/9186]  |
| 专题 | 青岛生物能源与过程研究所_绿色化学催化团队 |
| 作者单位 | 1.Tsingtao Brewery Co Ltd, State Key Lab Biol Fermentat Engn Beer, Qingdao 266100, Peoples R China 2.Chinese Acad Sci, Qingdao Inst Bioenergy & Bioproc Technol, Key Lab Biobased Mat, Qingdao 266101, Shandong, Peoples R China |
| 推荐引用方式 GB/T 7714 | Zhao, Fuhua,Hou, Tonggang,Wang, Jianxun,et al. Immobilization of proline-specific endoprotease on nonporous silica nanoparticles functionalized with amino group[J]. BIOPROCESS AND BIOSYSTEMS ENGINEERING,2017,40(1):1-7. |
| APA | Zhao, Fuhua.,Hou, Tonggang.,Wang, Jianxun.,Jiang, Yijun.,Huang, Shuxia.,...&Mu, Xindong.(2017).Immobilization of proline-specific endoprotease on nonporous silica nanoparticles functionalized with amino group.BIOPROCESS AND BIOSYSTEMS ENGINEERING,40(1),1-7. |
| MLA | Zhao, Fuhua,et al."Immobilization of proline-specific endoprotease on nonporous silica nanoparticles functionalized with amino group".BIOPROCESS AND BIOSYSTEMS ENGINEERING 40.1(2017):1-7. |
入库方式： OAI收割
来源：青岛生物能源与过程研究所
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