Sensitive detection of maltose and glucose based on dual enzyme-displayed bacteria electrochemical biosensor
文献类型：期刊论文
| 作者 | Liu, Aihua1,2,3; Lang, Qiaolin1,2,3,4 ; Liang, Bo4; Shi, Jianguo3
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| 刊名 | BIOSENSORS & BIOELECTRONICS
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| 出版日期 | 2017-01-15 |
| 卷号 | 87页码:25-30 |
| 关键词 | Glucoamylase displayed bacteria Glucose dehydrogenase displayed bacteria Maltose biosensor Glucose and maltose detection Saccharide analysis |
| 英文摘要 | Glucoamylase-displayed bacteria (GA-bacteria) and glucose dehydrogenase-displayed bacteria (GDH-bacteria) were co-immobilized on multi-walled carbon nanotubes (MWNTs) modified glassy carbon electrode (GCE) to construct GA-bacteria/GDH-bacteria/MWNTs/GCE biosensor. The biosensor was developed by optimizing the loading amount and the ratio of GA-bacteria to GDH-bacteria. The as prepared biosensor exhibited a wide dynamic range of 0.2-10 mM and a low detection limit of 0.1 mM maltose (S/N=3). The biosensor also had a linear response to glucose in the range of 0.1-2.0 mM and a low detection limit of 0.04 mM glucose (S/N=3). Interestingly, at the same concentration, glucose was 3.75-fold sensitive than that of maltose at the proposed biosensor. No interferences were observed for other possible mono- and disaccharides. The biosensor also demonstrated good long-term storage stability and repeatability. Further, using both GDH-bacteria/MWNTs/GCE biosensor and GA-bacteria/GDH-bacteria/MWNTs/GCE biosensor, glucose and maltose in real samples can be detected. Therefore, the proposed biosensor is capable of monitoring the food manufacturing and fermentation process. (C) 2016 Elsevier B.V. All rights reserved. |
| WOS标题词 | Science & Technology ; Life Sciences & Biomedicine ; Physical Sciences |
| 类目[WOS] | Biophysics ; Biotechnology & Applied Microbiology ; Chemistry, Analytical ; Electrochemistry ; Nanoscience & Nanotechnology |
| 研究领域[WOS] | Biophysics ; Biotechnology & Applied Microbiology ; Chemistry ; Electrochemistry ; Science & Technology - Other Topics |
| 关键词[WOS] | WHOLE-CELL BIOCATALYST ; XYLOSE DEHYDROGENASE ; BIOFUEL CELL ; CARBON NANOTUBES ; CONDUCTOMETRIC BIOSENSOR ; SURFACE DISPLAY ; FLOW-INJECTION ; ELECTRODE ; CONSTRUCTION ; OXIDASE |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000390499600005 |
| 源URL | [http://ir.qibebt.ac.cn/handle/337004/9194]  |
| 专题 | 青岛生物能源与过程研究所_环境资源化与水回用团队 |
| 作者单位 | 1.Qingdao Univ, Inst Biosensing, 308 Ningxia Rd, Qingdao 266071, Peoples R China 2.Qingdao Univ, Coll Chem & Chem Engn, 308 Ningxia Rd, Qingdao 266071, Peoples R China 3.Key Lab Biosensors Shangdong Prov, Jinan, Peoples R China 4.Chinese Acad Sci, Qingdao Inst Bioenergy & Bioproc Technol, Lab Biosensing, 189 Songling Rd, Qingdao 266101, Peoples R China |
| 推荐引用方式 GB/T 7714 | Liu, Aihua,Lang, Qiaolin,Liang, Bo,et al. Sensitive detection of maltose and glucose based on dual enzyme-displayed bacteria electrochemical biosensor[J]. BIOSENSORS & BIOELECTRONICS,2017,87:25-30. |
| APA | Liu, Aihua,Lang, Qiaolin,Liang, Bo,&Shi, Jianguo.(2017).Sensitive detection of maltose and glucose based on dual enzyme-displayed bacteria electrochemical biosensor.BIOSENSORS & BIOELECTRONICS,87,25-30. |
| MLA | Liu, Aihua,et al."Sensitive detection of maltose and glucose based on dual enzyme-displayed bacteria electrochemical biosensor".BIOSENSORS & BIOELECTRONICS 87(2017):25-30. |
入库方式： OAI收割
来源：青岛生物能源与过程研究所
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