New Surface-Enhanced Raman Sensing Chip Designed for On-Site Detection of Active Ricin in Complex Matrices Based on Specific Depurination
文献类型：期刊论文
| 作者 | Tang, Ji-jun; Sun, Jie-fang; Lui, Rui; Zhang, Zong-mian; Liu, Jing-fu; Xie, Jian-wei |
| 刊名 | ACS APPLIED MATERIALS & INTERFACES
 |
| 出版日期 | 2016-01-27 |
| 卷号 | 8期号:3页码:2449-2455 |
| 关键词 | gold nanoparticles surface-enhanced Raman spectroscopy active ricin depurination on-site detection |
| 英文摘要 | Quick and accurate on-site detection of active ricin has very important realistic significance in view of national security and defense. In this paper, optimized single-stranded oligodeoxynucleotides named poly(21dA), which function as a depurination substrate of active ricin, were screened and chemically attached on gold nanoparticles (AuNPs, similar to 100 nrn) via the Au-S bond [poly(21dA)-AuNPs]. Subsequently, poly(21dA) AuNPs were assembled on a dihydrogen lipoic-acid-modified Si wafer (SH-Si), thus forming the specific surface-enhanced Raman spectroscopy (SERS) chip [poly(21dA)-AuNPs@SH-Si] for depurination of active ricin. Under optimized conditions, active ricin could specifically hydrolyze multiple adenines from poly(21dA) on the chip. This depurination-induced composition change could be conveniently monitored by measuring the distinct attenuation of the SERS signature corresponding to adenine. To improve sensitivity of this method, a silver nanoshell was deposited on post-reacted poly(21dA)-AuNPs, which lowered the limit of detection to 8.9 ng mL(-1). The utility of this well controlled SERS chip was successfully demonstrated in food and biological matrices spiked with different concentrations of active ricin, thus showing to be very promising assay for reliable and rapid on-site detection of active ricin. |
| 源URL | [http://ir.rcees.ac.cn/handle/311016/35633]  |
| 专题 | 生态环境研究中心_城市与区域生态国家重点实验室 |
| 推荐引用方式 GB/T 7714 | Tang, Ji-jun,Sun, Jie-fang,Lui, Rui,et al. New Surface-Enhanced Raman Sensing Chip Designed for On-Site Detection of Active Ricin in Complex Matrices Based on Specific Depurination[J]. ACS APPLIED MATERIALS & INTERFACES,2016,8(3):2449-2455. |
| APA | Tang, Ji-jun,Sun, Jie-fang,Lui, Rui,Zhang, Zong-mian,Liu, Jing-fu,&Xie, Jian-wei.(2016).New Surface-Enhanced Raman Sensing Chip Designed for On-Site Detection of Active Ricin in Complex Matrices Based on Specific Depurination.ACS APPLIED MATERIALS & INTERFACES,8(3),2449-2455. |
| MLA | Tang, Ji-jun,et al."New Surface-Enhanced Raman Sensing Chip Designed for On-Site Detection of Active Ricin in Complex Matrices Based on Specific Depurination".ACS APPLIED MATERIALS & INTERFACES 8.3(2016):2449-2455. |
入库方式： OAI收割
来源：生态环境研究中心
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