Affinity capillary electrophoresis with laser induced fluorescence detection for thrombin analysis using nuclease-resistant RNA aptamers
文献类型：期刊论文
| 作者 | Hao, Lihua; Bai, Yunlong; Wang, Hailin; Zhao, Qiang |
| 刊名 | JOURNAL OF CHROMATOGRAPHY A
 |
| 出版日期 | 2016-12-09 |
| 卷号 | 1476期号:0页码:124-129 |
| 关键词 | RNA aptamer Thrombin Affinity binding Laser induced fluorescence Capillary electrophoresis 2 '-Fluoro modification |
| 英文摘要 | Aptamer affinity capillary electrophoresis coupled with laser-induced fluorescence (CE-LIF) combines the advantages of affinity aptamer, rapid CE separation, and high sensitivity detection. Here we reported an affinity CE-LIF assay for thrombin by using a fluorophore-labeled RNA aptamer containing 2'-fluoro modification in sugar rings of pyrimidine nucleotides (C and U) as affinity ligand. This RNA aptamer has high binding affinity, specificity and biostability. Thrombin at 0.2 nM was successfully detected. This RNA aptamer allowed for the detection of thrombin spiked in diluted human serum sample due to the nuclease resistance. The RNA aptamer has comparable binding affinity to a 29-mer DNA aptamer for thrombin, and the binding site of the RNA aptamer on thrombin partially overlaps with the binding site of the 29-mer DNA aptamer on thrombin. It shows the nuclease-resistant RNA aptamers are promising in assays for thrombin. (C) 2016 Elsevier B.V. All rights reserved. |
| 收录类别 | SCI |
| 源URL | [http://ir.rcees.ac.cn/handle/311016/35798]  |
| 专题 | 生态环境研究中心_环境化学与生态毒理学国家重点实验室 |
| 推荐引用方式 GB/T 7714 | Hao, Lihua,Bai, Yunlong,Wang, Hailin,et al. Affinity capillary electrophoresis with laser induced fluorescence detection for thrombin analysis using nuclease-resistant RNA aptamers[J]. JOURNAL OF CHROMATOGRAPHY A,2016,1476(0):124-129. |
| APA | Hao, Lihua,Bai, Yunlong,Wang, Hailin,&Zhao, Qiang.(2016).Affinity capillary electrophoresis with laser induced fluorescence detection for thrombin analysis using nuclease-resistant RNA aptamers.JOURNAL OF CHROMATOGRAPHY A,1476(0),124-129. |
| MLA | Hao, Lihua,et al."Affinity capillary electrophoresis with laser induced fluorescence detection for thrombin analysis using nuclease-resistant RNA aptamers".JOURNAL OF CHROMATOGRAPHY A 1476.0(2016):124-129. |
入库方式： OAI收割
来源：生态环境研究中心
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