Development and characterization of monoclonal antibodies against human aryl hydrocarbon receptor
文献类型：期刊论文
| 作者 | Tian, Wenjing; Pei, Xinhui; Xie, Heidi Qunhui; Xu, Sherry Li; Tian, Jijing; Hu, Qin; Xu, Haiming; Chen, Yangsheng; Fu, Hualing; Cao, Zhengyu |
| 刊名 | JOURNAL OF ENVIRONMENTAL SCIENCES
 |
| 出版日期 | 2016-01 |
| 卷号 | 39期号:0页码:165-174 |
| 关键词 | Aryl hydrocarbon receptor Monoclonal antibody Western blot Immunoprecipitation Indirect immunofluorescence assay |
| 英文摘要 | Aryl hydrocarbon receptor (AhR), a ligand-dependent nuclear receptor, is involved in a diverse spectrum of biological and toxicological effects. Due to the lack of three dimensional (3D) crystal or nuclear magnetic resonance structure, the mechanisms of these complex effects of AhR remain to be unclear. Also, commercial monoclonal antibodies (mAbs) against human AhR protein (hAhR), as alternative immunological tools, are very limited. Thus, in order to providemore tools for further studies on hAhR, we prepared twomAbs (1D6 and 4A6) against hAhR. The two newly generated mAbs specifically bound to amino acids 484-508 (located in transcription activation domain) and amino acids 201-215 (located in Per-ARNT-Sim domain) of hAhR, respectively. These epitopeswere newas compared with those of commercialmAbs. The mAbs were also characterized by enzyme-linked immunosorbent assay, western blot, immunoprecipitation and indirect immunofluorescence assay in different cell lines. The results showed that the twomAbs could recognize the linearized AhRs in six different human cell lines and a rat hepatoma cell line, as well as the hAhR with native conformations. We concluded that the newly generated mAbs could be employed in AhR-based bioassays for analysis of environmental contaminants, and held great potential for further revealing the spatial structure of AhR and its biological functions in future studies. (C) 2015 The Research Center for Eco-Environmental Sciences, Chinese Academy of Sciences. Published by Elsevier B.V. |
| 收录类别 | SCI |
| 源URL | [http://ir.rcees.ac.cn/handle/311016/35837]  |
| 专题 | 生态环境研究中心_环境化学与生态毒理学国家重点实验室 |
| 推荐引用方式 GB/T 7714 | Tian, Wenjing,Pei, Xinhui,Xie, Heidi Qunhui,et al. Development and characterization of monoclonal antibodies against human aryl hydrocarbon receptor[J]. JOURNAL OF ENVIRONMENTAL SCIENCES,2016,39(0):165-174. |
| APA | Tian, Wenjing.,Pei, Xinhui.,Xie, Heidi Qunhui.,Xu, Sherry Li.,Tian, Jijing.,...&Zhao, Bin.(2016).Development and characterization of monoclonal antibodies against human aryl hydrocarbon receptor.JOURNAL OF ENVIRONMENTAL SCIENCES,39(0),165-174. |
| MLA | Tian, Wenjing,et al."Development and characterization of monoclonal antibodies against human aryl hydrocarbon receptor".JOURNAL OF ENVIRONMENTAL SCIENCES 39.0(2016):165-174. |
入库方式： OAI收割
来源：生态环境研究中心
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