Insight into the Mechanisms of Combined Toxicity of Single-Walled Carbon Nanotubes and Nickel Ions in Macrophages: Role of P2X(7) Receptor
文献类型：期刊论文
| 作者 | Cui, Xuejing; Wan, Bin; Guo, Liang-Hong; Yang, Yu; Ren, Xiaomin |
| 刊名 | ENVIRONMENTAL SCIENCE & TECHNOLOGY
 |
| 出版日期 | 2016-11-15 |
| 卷号 | 50期号:22页码:12473-12483 |
| 英文摘要 | Coexistence of nanomaterials and environmental pollutants requires in-depth understanding of combined toxicity and underlying mechanism. In this work, we found that coexposure to the mixture of noncytotoxic level of single-walled carbon nanotubes (SWCNTs) (10 mu g/mL) and Ni2+ (20 mu M) induced significant cytotoxicity in macrophages. However, almost equal amount of intracellular Ni2+ was detected after Ni2+/SWCNT coexposure or Ni2+ single exposure, indicating no enhanced cellular uptake of Ni2+ occurred. SDS-PAGE analysis revealed 50% more SWCNTs retained in Ni2+/SWCNT exposed cells than that with SWCNT exposure alone, regardless of the exposure sequence (coexposure, Ni2+ pre- or post-treatment), suggesting inhibited SWCNT exocytosis by Ni2+. The increased cellular dose of SWCNTs could quantitatively account for the elevated toxicity of Ni2+/SWCNT mixture to cells. It was then found that agonist (ATP) and antagonist (o-ATP) of P2X(7)R could regulate intracellular SWCNT amount and the cytotoxicity accordingly. In addition, inhibition of P2X(7)R by P2X(7)-targeting siRNA diminished the inhibitory effect of Ni2+. It was therefore concluded that Ni2+ impeded SWCNT exocytosis by inhibiting P2X(7)R, leading to higher intracellular retention of SWCNTs and elevated cytotoxicity. Our work identified exocytosis inhibition as an important mechanism for SWCNT/Ni2+ toxicity, and revealed the crucial role of P2X(7)R. in mediating such inhibitory effect. |
| 收录类别 | SCI |
| 源URL | [http://ir.rcees.ac.cn/handle/311016/35877]  |
| 专题 | 生态环境研究中心_环境化学与生态毒理学国家重点实验室 |
| 推荐引用方式 GB/T 7714 | Cui, Xuejing,Wan, Bin,Guo, Liang-Hong,et al. Insight into the Mechanisms of Combined Toxicity of Single-Walled Carbon Nanotubes and Nickel Ions in Macrophages: Role of P2X(7) Receptor[J]. ENVIRONMENTAL SCIENCE & TECHNOLOGY,2016,50(22):12473-12483. |
| APA | Cui, Xuejing,Wan, Bin,Guo, Liang-Hong,Yang, Yu,&Ren, Xiaomin.(2016).Insight into the Mechanisms of Combined Toxicity of Single-Walled Carbon Nanotubes and Nickel Ions in Macrophages: Role of P2X(7) Receptor.ENVIRONMENTAL SCIENCE & TECHNOLOGY,50(22),12473-12483. |
| MLA | Cui, Xuejing,et al."Insight into the Mechanisms of Combined Toxicity of Single-Walled Carbon Nanotubes and Nickel Ions in Macrophages: Role of P2X(7) Receptor".ENVIRONMENTAL SCIENCE & TECHNOLOGY 50.22(2016):12473-12483. |
入库方式： OAI收割
来源：生态环境研究中心
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