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Nuclear m(6)A Reader YTHDC1 Regulates mRNA Splicing
文献类型：期刊论文
| 作者 | Xiao, Wen; Adhikari, Samir; Dahal, Ujwal; Chen, Yu-Sheng; Hao, Ya-Juan; Sun, Bao-Fa; Sun, Hui-Ying; Li, Ang; Ping, Xiao-Li; Lai, Wei-Yi |
| 刊名 | MOLECULAR CELL
 |
| 出版日期 | 2016-02-18 |
| 卷号 | 61期号:4页码:507-519 |
| 英文摘要 | The regulatory role of N-6-methyladenosine (m(6)A) and its nuclear binding protein YTHDC1 in premRNA splicing remains an enigma. Here we show that YTHDC1 promotes exon inclusion in targeted mRNAs through recruiting pre-mRNA splicing factor SRSF3 (SRp20) while blocking SRSF10 (SRp38) mRNA binding. Transcriptome assay with PARCLIP-seq analysis revealed that YTHDC1-regulated exon-inclusion patterns were similar to those of SRSF3 but opposite of SRSF10. In vitro pull-down assay illustrated a competitive binding of SRSF3 and SRSF10 to YTHDC1. Moreover, YTHDC1 facilitates SRSF3 but represses SRSF10 in their nuclear speckle localization, RNA-binding affinity, and associated splicing events, dysregulation of which, as the result of YTHDC1 depletion, can be restored by reconstitution with wild-type, but not m(6)A-bindingdefective, YTHDC1. Our findings provide the direct evidence that m6A reader YTHDC1 regulates mRNA splicing through recruiting and modulating premRNA splicing factors for their access to the binding regions of targeted mRNAs. |
| 收录类别 | SCI |
| 源URL | [http://ir.rcees.ac.cn/handle/311016/35905]  |
| 专题 | 生态环境研究中心_环境化学与生态毒理学国家重点实验室 |
| 推荐引用方式 GB/T 7714 | Xiao, Wen,Adhikari, Samir,Dahal, Ujwal,et al. Nuclear m(6)A Reader YTHDC1 Regulates mRNA Splicing[J]. MOLECULAR CELL,2016,61(4):507-519. |
| APA | Xiao, Wen.,Adhikari, Samir.,Dahal, Ujwal.,Chen, Yu-Sheng.,Hao, Ya-Juan.,...&Yang, Yun-Gui.(2016).Nuclear m(6)A Reader YTHDC1 Regulates mRNA Splicing.MOLECULAR CELL,61(4),507-519. |
| MLA | Xiao, Wen,et al."Nuclear m(6)A Reader YTHDC1 Regulates mRNA Splicing".MOLECULAR CELL 61.4(2016):507-519. |
入库方式： OAI收割
来源：生态环境研究中心
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