T-screen and yeast assay for the detection of the thyroid-disrupting activities of cadmium, mercury, and zinc
文献类型：期刊论文
| 作者 | Li, Jian; Liu, Yun; Kong, Dongdong; Ren, Shujuan; Li, Na |
| 刊名 | ENVIRONMENTAL SCIENCE AND POLLUTION RESEARCH
 |
| 出版日期 | 2016-05 |
| 卷号 | 23期号:10页码:9843-9851 |
| 关键词 | In vitro bioassay Toxicmetals Thyroid receptor Thyroid-disrupting activity |
| 英文摘要 | In the present study, a two-hybrid yeast bioassay and a T-screen were used to screen for the thyroid receptor (TR)-disrupting activity of select metallic compounds (CdCl2, ZnCl2, HgCl2, CuSO4, MnSO4, and MgSO4). The results reveal that none of the tested metallic compounds showed TR-agonistic activity, whereas ZnCl2, HgCl2, and CdCl2 demonstrated TR antagonism. For the yeast assay, the dose-response relationship of these metallic compounds was established, and the concentrations producing 20 % of the maximum effect of ZnCl2, HgCl2, and CdCl2 were 9.1 x 10(-5), 3.2 x 10(-6), and 1.2 x 10(-6) mol/L, respectively. The T-screen also supported the finding that ZnCl2, HgCl2, and CdCl2 decreased the cell proliferation at concentrations ranging from 10(-6) to 10(-4) mol/L. Furthermore, the thyroid-disrupting activity of metallic compounds in environmental water samples collected from the Guanting Reservoir, Beijing, China was evaluated. Solid-phase extraction was used to separate the organic extracts, and a modified two-hybrid yeast bioassay revealed that the metallic compounds in the water samples could affect thyroid hormone-induced signaling by decreasing the binding of the thyroid hormone. The addition of ethylenediaminetetraacetic acid (30 mg/L) could eliminate the effects. Thus, the cause(s) of the thyroid toxicity in the water samples appeared to be partly related to the metallic compounds. |
| 收录类别 | SCI |
| 源URL | [http://ir.rcees.ac.cn/handle/311016/36085]  |
| 专题 | 生态环境研究中心_环境水质学国家重点实验室 |
| 推荐引用方式 GB/T 7714 | Li, Jian,Liu, Yun,Kong, Dongdong,et al. T-screen and yeast assay for the detection of the thyroid-disrupting activities of cadmium, mercury, and zinc[J]. ENVIRONMENTAL SCIENCE AND POLLUTION RESEARCH,2016,23(10):9843-9851. |
| APA | Li, Jian,Liu, Yun,Kong, Dongdong,Ren, Shujuan,&Li, Na.(2016).T-screen and yeast assay for the detection of the thyroid-disrupting activities of cadmium, mercury, and zinc.ENVIRONMENTAL SCIENCE AND POLLUTION RESEARCH,23(10),9843-9851. |
| MLA | Li, Jian,et al."T-screen and yeast assay for the detection of the thyroid-disrupting activities of cadmium, mercury, and zinc".ENVIRONMENTAL SCIENCE AND POLLUTION RESEARCH 23.10(2016):9843-9851. |
入库方式： OAI收割
来源：生态环境研究中心
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