Multiplexed detection of lung cancer biomarkers based on quantum dots and microbeads
文献类型：期刊论文
| 作者 | Wu, Simin1; Liu, Lifen1; Li, Gong1; Jing, Fengxiang1; Mao, Hongju1; Jin, Qinghui1; Zhai, Wanyin3; Zhang, Hongfeng2; Zhao, Jianlong1; Jia, Chunping1 |
| 刊名 | TALANTA
 |
| 出版日期 | 2016-08-15 |
| 卷号 | 156页码:48-54 |
| 关键词 | Quantum dots Multiplexed detection Lung cancer Biomarker Immunoassay |
| 英文摘要 | We have developed a multiplexed fluoroimmunoassay of three lung cancer biomarkers based on multicolor quantum dots (QDs) as detection elements and micro-magnetic beads as immune carriers. QDs have the ability to simplify multiplexed analysis. In our method, the fluorescent signals derived from three cross-talk-free QD conjugated probes with emission maxima at 525, 585 and 625 nm could be analyzed to determine the concentrations of the target proteins. With this system, fragments of cytokeratin 19 (CYRFA 21-1), carcinoembryonic antigen (CEA), and neuron-specific enolase (NSE), were simultaneously detected in a single sample with a low detection limit down to the 1.0 ng/mL level (364 pg/mL for CYRFA 21-1, 38 pg/mL for CEA, 370 pg/mL for NSE in a single detection). Additional advantages of the presented method include ease of operation, low cost, and a very low sample volume (20 mu L). (C) 2016 Elsevier B.V. All rights reserved. |
| WOS标题词 | Science & Technology ; Physical Sciences |
| 类目[WOS] | Chemistry, Analytical |
| 研究领域[WOS] | Chemistry |
| 关键词[WOS] | SUSPENSION ARRAY TECHNOLOGY ; PROSTATE-CANCER ; IMMUNOASSAY ; BIOSENSOR ; SERUM ; DNA |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000378182900008 |
| 源URL | [http://ir.sic.ac.cn/handle/331005/22952]  |
| 专题 | 上海硅酸盐研究所_生物材料与组织工程研究中心_期刊论文 |
| 作者单位 | 1.Chinese Acad Sci, Shanghai Inst Microsyst & Informat Technol, State Key Lab Transducer Technol, 865 Changning Rd, Shanghai 200050, Peoples R China 2.E China Normal Univ, Sch Life Sci, Shanghai 200241, Peoples R China 3.Chinese Acad Sci, Shanghai Inst Ceram, Biomat & Tissue Engn Res Ctr, Shanghai 200050, Peoples R China |
| 推荐引用方式 GB/T 7714 | Wu, Simin,Liu, Lifen,Li, Gong,et al. Multiplexed detection of lung cancer biomarkers based on quantum dots and microbeads[J]. TALANTA,2016,156:48-54. |
| APA | Wu, Simin.,Liu, Lifen.,Li, Gong.,Jing, Fengxiang.,Mao, Hongju.,...&Jia, Chunping.(2016).Multiplexed detection of lung cancer biomarkers based on quantum dots and microbeads.TALANTA,156,48-54. |
| MLA | Wu, Simin,et al."Multiplexed detection of lung cancer biomarkers based on quantum dots and microbeads".TALANTA 156(2016):48-54. |
入库方式： OAI收割
来源：上海硅酸盐研究所
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