CHIP involves in non-small cell lung cancer prognosis through VEGF pathway
文献类型：期刊论文
| 作者 | Qian, TT; Wang, J; Wang, QF; Liu, YC; Yu, SJ; Wang, ZQ; Sun, DM; Wang, SL |
| 刊名 | BIOMEDICINE & PHARMACOTHERAPY
 |
| 出版日期 | 2016 |
| 卷号 | 83页码:271-276 |
| 关键词 | NSCLC CHIP VEGF-VEGFR2 Prognosis |
| ISSN号 | 0753-3322 |
| 通讯作者 | Qian, TT (reprint author), Tongji Univ, Sch Life Sci & Technol, 1239 Siping Rd, Shanghai 200092, Peoples R China. |
| 英文摘要 | Aim: CHIP (c-terminal Hsp70-interacting protein) is an E3 ligase playing vital roles in various cancers. The VEGF pathway has become an important therapeutic target in non-small cell lung cancer (NSCLC). However, little is known about the role of CHIP and the relationship between CHIP and VEGF-VEGFR2 (VEGF receptor 2) pathway in NSCLC. In this study we aimed to investigate the clinical function of CHIP in NSCLC and explore the relevant regulatory mechanism. Methods: QRT-PCR was performed to detect CHIP expression in NSCLC tissues. The association of CHIP expression and clinical parameters was analyzed using the Chi-square test. Kaplan-Meier and Cox analyses were performed to identify the role of CHIP in the prognosis of NSCLC patients. ELISA test was used to detect the VEGF secretion of NSCLC cells and western blot were used to detected the protein expression of VEGFR2 in NSCLC cells. Results: and the results revealed that CHIP expression was decreased in NSCLC tissues and significantly correlated with clinical stages, lymph node metastasis and distant metastasis (P < 0.05). Moreover, Kaplan-Meier and Cox regression analyses showed that patients with negative expression of CHIP had a shorter survival time and CHIP could be an independent prognostic biomarker. In addition, ELISA tests showed that CHIP negatively regulated the secretion level of VEGF. Furthermore, western blot assay indicated that the VEGFR2 protein level was reduced after CHIP over-expression. Conclusions: Taken together, our findings demonstrate for the first time that CHIP may serve as a promising prognostic biomarker for NSCLC patients and it may be involved in NSCLC angiogenesis through regulating VEGF secretion and expression of VEGFR2. (C) 2016 Published by Elsevier Masson SAS. |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000390433400034 |
| 源URL | [http://ir.sinap.ac.cn/handle/331007/26448]  |
| 专题 | 上海应用物理研究所_中科院上海应用物理研究所2011-2017年 |
| 推荐引用方式 GB/T 7714 | Qian, TT,Wang, J,Wang, QF,et al. CHIP involves in non-small cell lung cancer prognosis through VEGF pathway[J]. BIOMEDICINE & PHARMACOTHERAPY,2016,83:271-276. |
| APA | Qian, TT.,Wang, J.,Wang, QF.,Liu, YC.,Yu, SJ.,...&Wang, SL.(2016).CHIP involves in non-small cell lung cancer prognosis through VEGF pathway.BIOMEDICINE & PHARMACOTHERAPY,83,271-276. |
| MLA | Qian, TT,et al."CHIP involves in non-small cell lung cancer prognosis through VEGF pathway".BIOMEDICINE & PHARMACOTHERAPY 83(2016):271-276. |
入库方式： OAI收割
来源：上海应用物理研究所
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