High Resolution Imaging of Single-Molecule Immunoglobulin G Antibodies with Atomic Force Microscopy in Liquid
文献类型：期刊论文
| 作者 | Zhao, ZJ; Zhang, P; Yang, JX; Hao, CC; Zhou, XF; Li, B |
| 刊名 | CHINESE JOURNAL OF ANALYTICAL CHEMISTRY
 |
| 出版日期 | 2016 |
| 卷号 | 44期号:8页码:1215-1220 |
| 关键词 | Atomic force microscopy Single-molecule High-resolution imaging Immunoglobulin G DNA origami |
| ISSN号 | 0253-3820 |
| 通讯作者 | Li, B (reprint author), Chinese Acad Sci, Shanghai Inst Appl Phys, Div Phys Biol, Shanghai Synchrotron Radiat Facil,Key Lab Interfa, Shanghai 201800, Peoples R China. ; Li, B (reprint author), Chinese Acad Sci, Shanghai Inst Appl Phys, Bioimaging Ctr, Shanghai Synchrotron Radiat Facil,Key Lab Interfa, Shanghai 201800, Peoples R China. |
| 英文摘要 | Atomic force microscopy (AFM) is one of important tools for studying the structure and function of biomolecules due to its nanoscale capability. A well prepared sample was the primary key issue for high. resolution imaging macromolecules. Here, by using self. assembly technology of DNA origami, antigens were modified on DNA origami. Afterward, antibodies reacted specifically with antigens by a molecular recognition way. Then, a nanostructure formed from DNA origami and antigen. antibody complexes. By the aid of adsorption character of DNA origami, individual antibodies could selectively adsorb on mica surface. As a result, the ultrastructural morphology image of a single IgG molecule, the characteristic "Y. shaped. domains, was resolved in liquid. The way for adsorbing biomolecules on the mica surface in a liquid environment was simple and convenient, and it may be useful to detect and measure biomoleculers at the single-molecule level with AFM. |
| 收录类别 | SCI |
| 语种 | 中文 |
| WOS记录号 | WOS:000389035300007 |
| 源URL | [http://ir.sinap.ac.cn/handle/331007/26528]  |
| 专题 | 上海应用物理研究所_中科院上海应用物理研究所2011-2017年 |
| 推荐引用方式 GB/T 7714 | Zhao, ZJ,Zhang, P,Yang, JX,et al. High Resolution Imaging of Single-Molecule Immunoglobulin G Antibodies with Atomic Force Microscopy in Liquid[J]. CHINESE JOURNAL OF ANALYTICAL CHEMISTRY,2016,44(8):1215-1220. |
| APA | Zhao, ZJ,Zhang, P,Yang, JX,Hao, CC,Zhou, XF,&Li, B.(2016).High Resolution Imaging of Single-Molecule Immunoglobulin G Antibodies with Atomic Force Microscopy in Liquid.CHINESE JOURNAL OF ANALYTICAL CHEMISTRY,44(8),1215-1220. |
| MLA | Zhao, ZJ,et al."High Resolution Imaging of Single-Molecule Immunoglobulin G Antibodies with Atomic Force Microscopy in Liquid".CHINESE JOURNAL OF ANALYTICAL CHEMISTRY 44.8(2016):1215-1220. |
入库方式： OAI收割
来源：上海应用物理研究所
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