Ion-Mediated Polymerase Chain Reactions Performed with an Electronically Driven Microfluidic Device
文献类型：期刊论文
| 作者 | Zhang, Y; Li, Q; Guo, LJ; Huang, Q; Shi, JY; Yang, Y; Liu, DS; Fan, CH |
| 刊名 | ANGEWANDTE CHEMIE-INTERNATIONAL EDITION
 |
| 出版日期 | 2016 |
| 卷号 | 55期号:40页码:12450-12454 |
| 关键词 | dna amplification isothermal PCR microfluidic devices pH cycling pH-stats |
| ISSN号 | 1433-7851 |
| 通讯作者 | Fan, CH (reprint author), Chinese Acad Sci, Shanghai Inst Appl Phys, Div Phys Biol, Shanghai Synchrotron Radiat Facil,CAS Key Lab Int, Shanghai 201800, Peoples R China. ; Fan, CH (reprint author), Chinese Acad Sci, Shanghai Inst Appl Phys, Bioimaging Ctr, Shanghai Synchrotron Radiat Facil,CAS Key Lab Int, Shanghai 201800, Peoples R China. ; Liu, DS (reprint author), Tsinghua Univ, Dept Chem, Minist Educ, Key Lab Organ Optoelect & Mol Engn, Beijing 100084, Peoples R China. |
| 英文摘要 | The polymerase chain reaction (PCR) is a powerful method for exponentially amplifying very low amounts of target DNA from genetic, clinical, and forensic samples. However, the heating and cooling steps in PCR largely hamper the miniaturization of thermocyclers for on-site detection of pathogens and point-of-care tests. Herein, we devise an ion-mediated PCR (IM-PCR) strategy by exploiting ion-induced DNA denaturation/renaturation cycles. DNA duplexes are effectively denatured in alkaline solutions; whereas, the denatured single-stranded DNA strands readily reform duplexes at neutral pH. By using an integrated micro-chip that can programmably control the solution pH simply switching the potential in a range of several hundred millivolts, we can trigger IM-PCR at a constant temperature. Analogously to thermal cycling, 30 cycles of pH-induced denaturation/renaturation were used to amplify protein DNA fragments as confirmed by DNA sequencing. We anticipate that this portable, low-cost, and scalable IM-PCR holds great promise for widespread biological, clinical, and environmental applications. |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000384713700057 |
| 源URL | [http://ir.sinap.ac.cn/handle/331007/26551]  |
| 专题 | 上海应用物理研究所_中科院上海应用物理研究所2011-2017年 |
| 推荐引用方式 GB/T 7714 | Zhang, Y,Li, Q,Guo, LJ,et al. Ion-Mediated Polymerase Chain Reactions Performed with an Electronically Driven Microfluidic Device[J]. ANGEWANDTE CHEMIE-INTERNATIONAL EDITION,2016,55(40):12450-12454. |
| APA | Zhang, Y.,Li, Q.,Guo, LJ.,Huang, Q.,Shi, JY.,...&Fan, CH.(2016).Ion-Mediated Polymerase Chain Reactions Performed with an Electronically Driven Microfluidic Device.ANGEWANDTE CHEMIE-INTERNATIONAL EDITION,55(40),12450-12454. |
| MLA | Zhang, Y,et al."Ion-Mediated Polymerase Chain Reactions Performed with an Electronically Driven Microfluidic Device".ANGEWANDTE CHEMIE-INTERNATIONAL EDITION 55.40(2016):12450-12454. |
入库方式： OAI收割
来源：上海应用物理研究所
浏览0
下载0
收藏0
其他版本
除非特别说明，本系统中所有内容都受版权保护，并保留所有权利。