Superresolution imaging of telomeres with continuous wave stimulated emission depletion (STED) microscope
文献类型：期刊论文
| 作者 | Wang, SP; Deng, SH; Cai, XQ; Hou, SG; Li, JJ; Gao, ZS; Li, J; Wang, LH; Fan, CH |
| 刊名 | SCIENCE CHINA-CHEMISTRY
 |
| 出版日期 | 2016 |
| 卷号 | 59期号:11页码:1519-1524 |
| 关键词 | telomere superresolution imaging stimulated emission depletion (STED) microscopy fluorescence in situ hybridization (FISH) |
| ISSN号 | 1674-7291 |
| 通讯作者 | Li, J (reprint author), Chinese Acad Sci, CAS Key Lab Interfacial Phys & Technol, Shanghai Synchrotron Radiat Facil, Div Phys Biol,Shanghai Inst Appl Phys, Shanghai 201800, Peoples R China. ; Li, J (reprint author), Chinese Acad Sci, CAS Key Lab Interfacial Phys & Technol, Shanghai Synchrotron Radiat Facil, Bioimaging Ctr,Shanghai Inst Appl Phys, Shanghai 201800, Peoples R China. |
| 英文摘要 | The significant role of telomeres in cells has attracted much attention since they were discovered. Fluorescence imaging is an effective method to study subcellular structures like telomeres. However, the diffraction limit of traditional optical microscope hampers further investigation on them. Recent progress on superresolution fluorescence microscopy has broken this limit. In this work, we used stimulated emission depletion (STED) microscope to observe fluorescence-labeled telomeres in interphase cell nuclei. The results showed that the size of fluorescent puncta representing telomeres under the STED microscope was much smaller than that under the confocal microscope. Two adjacent telomeres were clearly separated via STED imaging, which could hardly be discriminated by confocal microscopy due to the diffraction limit. We conclude that STED microscope is a more powerful tool that enable us to obtain detailed information about telomeres. |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000387571100021 |
| 源URL | [http://ir.sinap.ac.cn/handle/331007/26640]  |
| 专题 | 上海应用物理研究所_中科院上海应用物理研究所2011-2017年 |
| 推荐引用方式 GB/T 7714 | Wang, SP,Deng, SH,Cai, XQ,et al. Superresolution imaging of telomeres with continuous wave stimulated emission depletion (STED) microscope[J]. SCIENCE CHINA-CHEMISTRY,2016,59(11):1519-1524. |
| APA | Wang, SP.,Deng, SH.,Cai, XQ.,Hou, SG.,Li, JJ.,...&Fan, CH.(2016).Superresolution imaging of telomeres with continuous wave stimulated emission depletion (STED) microscope.SCIENCE CHINA-CHEMISTRY,59(11),1519-1524. |
| MLA | Wang, SP,et al."Superresolution imaging of telomeres with continuous wave stimulated emission depletion (STED) microscope".SCIENCE CHINA-CHEMISTRY 59.11(2016):1519-1524. |
入库方式： OAI收割
来源：上海应用物理研究所
浏览0
下载0
收藏0
其他版本
除非特别说明，本系统中所有内容都受版权保护，并保留所有权利。