中国科学院机构知识库网格
Chinese Academy of Sciences Institutional Repositories Grid
Target-guided isolation of polar antioxidants from Abelmoschus esculentus (L). Moench by high-speed counter-current chromatography method coupled with wavelength switching and extrusion elution mode

文献类型:期刊论文

作者Wang, RH; Liu, Q; Wu, ZL; Wang, ML; Chen, XQ
刊名JOURNAL OF SEPARATION SCIENCE
出版日期2016
卷号39期号:20页码:3983-3989
关键词Abelmoschus esculentus (L). Moench Antioxidants Extrusion elution mode High-speed counter-current chromatography Wavelength switching
ISSN号1615-9306
通讯作者Chen, XQ (reprint author), Cent S Univ, Coll Chem & Chem Engn, Changsha 410083, Hunan, Peoples R China.
英文摘要An off-line two-dimensional high-speed counter-current chromatography strategy combined with the wavelength switching technique and extrusion elution mode was successfully developed and applied to the isolation of polar antioxidants from Abelmoschus esculentus (L). Moench. Target-guided by the result of 2,2-diphenyl-1-picrylhydrazyl screening assay, four antioxidants were obtained with purities over 90% through orthogonal high-speed counter-current chromatography separation. UV spectroscopy, mass spectrometry and H-1 NMR spectroscopy were employed to identify their structures, which were assigned as L-tryptophan, quercetin-3-O-sophoroside, 5,7,3 ',4 '-tetrahydroxyflavonol-3-O-[beta-D-rhamnopyranosil-(1 -> 2)]-beta-D-glucopyranoside, and quercetin-3-O-glucoside. Each monomer exhibited significant antioxidant activity. The results demonstrated that proposed method could be an effective approach to isolate bioactive compounds from complex natural products.
收录类别SCI
语种英语
WOS记录号WOS:000389045100015
源URL[http://ir.sinap.ac.cn/handle/331007/26648]  
专题上海应用物理研究所_中科院上海应用物理研究所2011-2017年
推荐引用方式
GB/T 7714
Wang, RH,Liu, Q,Wu, ZL,et al. Target-guided isolation of polar antioxidants from Abelmoschus esculentus (L). Moench by high-speed counter-current chromatography method coupled with wavelength switching and extrusion elution mode[J]. JOURNAL OF SEPARATION SCIENCE,2016,39(20):3983-3989.
APA Wang, RH,Liu, Q,Wu, ZL,Wang, ML,&Chen, XQ.(2016).Target-guided isolation of polar antioxidants from Abelmoschus esculentus (L). Moench by high-speed counter-current chromatography method coupled with wavelength switching and extrusion elution mode.JOURNAL OF SEPARATION SCIENCE,39(20),3983-3989.
MLA Wang, RH,et al."Target-guided isolation of polar antioxidants from Abelmoschus esculentus (L). Moench by high-speed counter-current chromatography method coupled with wavelength switching and extrusion elution mode".JOURNAL OF SEPARATION SCIENCE 39.20(2016):3983-3989.

入库方式: OAI收割

来源:上海应用物理研究所

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