The Role of miR-34a in Tritiated Water Toxicity in Human Umbilical Vein Endothelial Cells
文献类型：期刊论文
| 作者 | Cui, FM; Liu, L; Zheng, LL; Bao, GL; Tu, Y; Sun, L; Zhu, W; Cao, JP; Zhou, PK; Chen, Q |
| 刊名 | DOSE-RESPONSE
 |
| 出版日期 | 2016 |
| 卷号 | 14期号:2页码:- |
| 关键词 | MiR-34a tritiated water DNA damage c-myc |
| ISSN号 | 1559-3258 |
| 通讯作者 | Chen, Q (reprint author), Soochow Univ, Sch Radiat Med & Protect, Dept Radiat Med, Coll Med, Suzhou 215123, Peoples R China. ; He, YM (reprint author), Soochow Univ, Dept Cardiol, Affiliated Hosp 1, Suzhou 215123, Peoples R China. |
| 英文摘要 | In this work, we investigated the toxic effects of tritiated water (HTO) on the cardiovascular system. We examined the role of microRNA-34a (miR-34a) in DNA damage and repair in human umbilical vein endothelial cells (HUVECs) exposed to HTO. Cell proliferation capacity was evaluated by cell counting, and miR-34a expression was detected using quantitative PCR (QT-PCR). The Comet assay and -H2AX immunostaining were used to measure DNA double-strand breaks (DSBs). Reverse transcription polymerase chain reaction was used to measure the expression level of c-myc messenger RNA (mRNA). The cells exposed to HTO showed significantly lower proliferation than the control cells over 3 days. The DNA damage in the HTO group was more severe than that in the control group, at each time point examined. The expression of miR-34a mimics caused increased DNA DSBs whereas that of the miR-34a inhibitor caused decreased DNA DSBs. The proliferation viability was the opposite for the miR-34a mimics and inhibitor groups. The expression levels of c-myc mRNA in cells transfected with miR-34a mimics were lower than that in cells transfected with the miR-34a-5p inhibitor, at 0.5 hours and 2 hours after transfection. In summary, miR-34a mediates HTO toxicity in HUVECs by downregulating the expression of c-myc. |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000380136800001 |
| 源URL | [http://ir.sinap.ac.cn/handle/331007/26657]  |
| 专题 | 上海应用物理研究所_中科院上海应用物理研究所2011-2017年 |
| 推荐引用方式 GB/T 7714 | Cui, FM,Liu, L,Zheng, LL,et al. The Role of miR-34a in Tritiated Water Toxicity in Human Umbilical Vein Endothelial Cells[J]. DOSE-RESPONSE,2016,14(2):-. |
| APA | Cui, FM.,Liu, L.,Zheng, LL.,Bao, GL.,Tu, Y.,...&He, YM.(2016).The Role of miR-34a in Tritiated Water Toxicity in Human Umbilical Vein Endothelial Cells.DOSE-RESPONSE,14(2),-. |
| MLA | Cui, FM,et al."The Role of miR-34a in Tritiated Water Toxicity in Human Umbilical Vein Endothelial Cells".DOSE-RESPONSE 14.2(2016):-. |
入库方式： OAI收割
来源：上海应用物理研究所
浏览0
下载0
收藏0
其他版本
除非特别说明，本系统中所有内容都受版权保护，并保留所有权利。