In Situ Saccharification of Cellulose using a Cellulase Mixture and Supplemental beta-glucosidase in Aqueous-Ionic Liquid Media
文献类型：期刊论文
| 作者 | Zhu, Changhui ; Guo, Feng ; Guo, Xiaoqian ; Li, Xingkang |
| 刊名 | BIORESOURCES
 |
| 出版日期 | 2017 |
| 卷号 | 11期号:4页码:9068-9078 |
| 关键词 | In situ saccharification Aqueous-ionic liquid media Cellulase Cellobiase Synergism |
| 中文摘要 | In situ enzymatic saccharification is one promising approach to efficiently convert non-food biomass to glucose as a platform chemical. The goal of the present research was to illustrate the catalytic behaviors and deactivation mechanisms of cellulase and cellobiase in an aqueous-ionic liquid (IL) media. The hydrolysis reaction rate was considerably increased because of the increased porosity and reduced crystallinity of the substrate. To lower the inhibitory effect of accumulated cellobiose on cellulase, a multi-enzyme synergetic system was explored. Compared with the single cellulase saccharification, addition of cellobiase resulted in a substantial increase in total reducing sugars (TRS) yield (88.0% vs. 59.1%) and glucose yield (40.6% vs. 28.9%) when the activity unit ratio of cellobiase/cellulase was 0.95. Cellulase showed a higher stability in aqueous 1-ethyl-3-methylimidazolium acetate ([EMIM]Ac) media. However, the activity of cellobiase quickly decreased, by 66.5%, after 6 h of incubation. |
| 收录类别 | SCI |
| 语种 | 英语 |
| 公开日期 | 2017-03-10 |
| 源URL | [http://ir.xtbg.org.cn/handle/353005/10321]  |
| 专题 | 西双版纳热带植物园_生物能源研究组 |
| 推荐引用方式 GB/T 7714 | Zhu, Changhui,Guo, Feng,Guo, Xiaoqian,et al. In Situ Saccharification of Cellulose using a Cellulase Mixture and Supplemental beta-glucosidase in Aqueous-Ionic Liquid Media[J]. BIORESOURCES,2017,11(4):9068-9078. |
| APA | Zhu, Changhui,Guo, Feng,Guo, Xiaoqian,&Li, Xingkang.(2017).In Situ Saccharification of Cellulose using a Cellulase Mixture and Supplemental beta-glucosidase in Aqueous-Ionic Liquid Media.BIORESOURCES,11(4),9068-9078. |
| MLA | Zhu, Changhui,et al."In Situ Saccharification of Cellulose using a Cellulase Mixture and Supplemental beta-glucosidase in Aqueous-Ionic Liquid Media".BIORESOURCES 11.4(2017):9068-9078. |
入库方式： OAI收割
来源：西双版纳热带植物园
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