Improving adjuvanticity of quaternized chitosan-based microgels for H5N1 split vaccine by tailoring the particle properties to achieve antigen dose sparing effect
文献类型:期刊论文
作者 | Wang, Yue-Qi1,2; Fan, Qing-Ze1,2; Liu, Yan1,2; Yue, Hua1; Ma, Xiao-Wei3; Wu, Jie1; Ma, Guang-Hui1,4; Su, Zhi-Guo1 |
刊名 | INTERNATIONAL JOURNAL OF PHARMACEUTICS |
出版日期 | 2016-12-30 |
卷号 | 515期号:1-2页码:84-93 |
ISSN号 | 0378-5173 |
关键词 | Quaternized chitosan microgels Hydrogel Dose sparing effect Adjuvant H5N1 split vaccine |
英文摘要 | In this study, we developed the quaternized chitosan microgels without chemical crosslinking as an adjuvant of H5N1 split vaccine. The microgels with pH-sensitivity, positive surface charge and good biocompatibility, have been demonstrated in favor of enhancing both humoral and cellular immune response. However, the detailed mechanism of the chitosan-based microgels to enhance antigen specific immune responses remains unclear. Therefore, we prepared the quaternized chitosan microgels with well defined quaternization degrees (QDs, 20-80%) and particle sizes (800 nm-5 mu m) by the premix membrane emulsification technique, and investigated the effect of quaternization degree (QD) and size on the adjuvanticity of microgels. Results suggested that microgels with relatively smaller size (807 nm) and moderate quaternization degree (QD 41% and 60%) were favorable for a maximum immune response. The mechanism was studied and explained by examining the characteristics of microgels and investigating the stimulation of bone-marrow derived dendritic cells (BMDCs). Moreover, they induced significantly stronger immune responses at lower antigen doses (known as antigen sparing effect) compared to aluminum adjuvant. These data indicated that a maximum immune response can be achieved by controlling properties of chitosan microgels, which also could serve as a significant guidance for rational design of chitosan-based particle adjuvant. (C) 2016 Elsevier B.V. All rights reserved. |
WOS标题词 | Science & Technology ; Life Sciences & Biomedicine |
类目[WOS] | Pharmacology & Pharmacy |
研究领域[WOS] | Pharmacology & Pharmacy |
关键词[WOS] | DENDRITIC CELLS ; DRUG-DELIVERY ; INFLUENZA VACCINES ; IN-VITRO ; VIRUS ; IMMUNOGENICITY ; MICROPARTICLES ; FUTURE ; SAFETY ; ACTIVATION |
收录类别 | SCI |
语种 | 英语 |
WOS记录号 | WOS:000389150700009 |
源URL | [http://ir.ipe.ac.cn/handle/122111/21842] |
专题 | 过程工程研究所_生化工程国家重点实验室 |
作者单位 | 1.Chinese Acad Sci, Inst Proc Engn, Natl Key Lab Biochem Engn, Beijing, Peoples R China 2.Univ Chinese Acad Sci, Beijing, Peoples R China 3.Hualan Biol Engn Inc, Xinxiang, Henan, Peoples R China 4.Jiangsu Natl Synerget Innovat Ctr Adv Mat SICAM, Nanjing, Jiangsu, Peoples R China |
推荐引用方式 GB/T 7714 | Wang, Yue-Qi,Fan, Qing-Ze,Liu, Yan,et al. Improving adjuvanticity of quaternized chitosan-based microgels for H5N1 split vaccine by tailoring the particle properties to achieve antigen dose sparing effect[J]. INTERNATIONAL JOURNAL OF PHARMACEUTICS,2016,515(1-2):84-93. |
APA | Wang, Yue-Qi.,Fan, Qing-Ze.,Liu, Yan.,Yue, Hua.,Ma, Xiao-Wei.,...&Su, Zhi-Guo.(2016).Improving adjuvanticity of quaternized chitosan-based microgels for H5N1 split vaccine by tailoring the particle properties to achieve antigen dose sparing effect.INTERNATIONAL JOURNAL OF PHARMACEUTICS,515(1-2),84-93. |
MLA | Wang, Yue-Qi,et al."Improving adjuvanticity of quaternized chitosan-based microgels for H5N1 split vaccine by tailoring the particle properties to achieve antigen dose sparing effect".INTERNATIONAL JOURNAL OF PHARMACEUTICS 515.1-2(2016):84-93. |
入库方式: OAI收割
来源:过程工程研究所
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