Chemical Coating Polysaccharide on Gigaporous Polystyrene Microspheres as a High-Speed Protein Chromatography Matrix
文献类型:期刊论文
作者 | Qu, Jian-Bo1; Xu, Yu-Liang1; Liu, Jun-Yi1; Li, Shi-Hai1; Zhou, Wei-Qing2; Liu, Jian-Guo1 |
刊名 | POLYMER ENGINEERING AND SCIENCE |
出版日期 | 2016-12-01 |
卷号 | 56期号:12页码:1407-1414 |
ISSN号 | 0032-3888 |
英文摘要 | Permanent polysaccharide coating on gigaporous polystyrene (PS) microspheres was achieved through direct chemical grafting of dextran via a facile method, minimizing nonspecific adsorption of proteins and providing easily derivatizable groups on microspheres. Chloroacetylated PS microspheres (PS-Cl) were first developed through acetylation, and hydrophilic dextran was directly grafted to hydrophobic PS-Cl via a Williamson reaction (PS-Dex). The homogeneous side-bound dextran coating has the advantage of masking the hydrophobic surface of the PS microspheres, while maintains their original gigaporous structure. After grafting, the hydrophobic surface of the PS microspheres was effectively masked, and nonspecific protein adsorption was greatly reduced. Moreover, the hydroxyl-rich coating can be easily derivatized through classical chemical methods. A column packed with PS-Dex presented both good permeability and mechanical strength at high flow velocities of up to 3612 cm/h. These results implied that PS-Dex should be an ideal matrix for highspeed protein chromatography. (C) 2016 Society of Plastics Engineers. |
WOS标题词 | Science & Technology ; Technology ; Physical Sciences |
类目[WOS] | Engineering, Chemical ; Polymer Science |
研究领域[WOS] | Engineering ; Polymer Science |
关键词[WOS] | PERFUSION CHROMATOGRAPHY ; LIQUID-CHROMATOGRAPHY ; ADSORPTION ; PARTICLES ; SEPARATION ; SUPPORTS ; DEXTRAN ; PHASE |
收录类别 | SCI |
语种 | 英语 |
WOS记录号 | WOS:000393121400011 |
源URL | [http://ir.ipe.ac.cn/handle/122111/21903] |
专题 | 过程工程研究所_生化工程国家重点实验室 |
作者单位 | 1.China Univ Petr East China, Ctr Bioengn & Biotechnol, State Key Lab Heavy Oil Proc, Qingdao 266580, Peoples R China 2.Chinese Acad Sci, Inst Proc Engn, Natl Key Lab Biochem Engn, Beijing 100190, Peoples R China |
推荐引用方式 GB/T 7714 | Qu, Jian-Bo,Xu, Yu-Liang,Liu, Jun-Yi,et al. Chemical Coating Polysaccharide on Gigaporous Polystyrene Microspheres as a High-Speed Protein Chromatography Matrix[J]. POLYMER ENGINEERING AND SCIENCE,2016,56(12):1407-1414. |
APA | Qu, Jian-Bo,Xu, Yu-Liang,Liu, Jun-Yi,Li, Shi-Hai,Zhou, Wei-Qing,&Liu, Jian-Guo.(2016).Chemical Coating Polysaccharide on Gigaporous Polystyrene Microspheres as a High-Speed Protein Chromatography Matrix.POLYMER ENGINEERING AND SCIENCE,56(12),1407-1414. |
MLA | Qu, Jian-Bo,et al."Chemical Coating Polysaccharide on Gigaporous Polystyrene Microspheres as a High-Speed Protein Chromatography Matrix".POLYMER ENGINEERING AND SCIENCE 56.12(2016):1407-1414. |
入库方式: OAI收割
来源:过程工程研究所
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