Specific N-glycan alterations are coupled in epithelial-mesenchymal transition induced by EGF in GE11 epithelial cells
文献类型：期刊论文
| 作者 | Xu, Qingsong1,2; Qu, Chen1; Wang, Wenjing3; Gu, Jianguo2; Du, Yuguang4; Song, Linsheng1 |
| 刊名 | CELL BIOLOGY INTERNATIONAL
 |
| 出版日期 | 2017-02-01 |
| 卷号 | 41期号:2页码:124-133 |
| ISSN号 | 1065-6995 |
| 关键词 | bisecting GlcNAc epidermal growth factor epithelial-mesenchymal transition metastasis N-glycan tumorigenesis |
| 英文摘要 | Epithelial-mesenchymal transition (EMT) is a phenomenon in cancer progression during which cancer cells undergo remarkable alteration acquiring highly invasive property. The aim of this study was to evaluate specific N-glycan alterations during EMT induced by epidermal growth factor (EGF) in GE11 epithelial cells. Herein, we demonstrated that EGF activated epidermal growth factor receptor (EGFR)/Akt/extracellular signal-regulated kinase (ERK) phosphorylation and promoted GE11 cell proliferation. Meanwhile, EGF stimulated the epithelial cells to undergo morphological alteration, destroying cell-cell inter-contact and exhibiting mesenchymal cells higher metastatic potential. A wound-healing assay showed the migratory ability increased 1.5-fold after EGF treatment. Moreover, the relative intensity of N-cadherin versus E-cadherin increased 2.6-fold, and the E-cadherin distribution in cell-cell junctions became jagged and faint after EGF incubation for 72h. Interestingly, the amounts of bisecting GlcNAc structure were dramatically declined, by contrast, the formation of 1,6 GlcNAc branches on cell surface was upregulated during EMT induced by EGF. To understand the roles of N-glycans in EGF-induced EMT, the cells were stably transfected with N-acetylglucosaminyltransferase III (GnT-III), which catalyzes the bisecting GlcNAc structure formation. As the markers for EMT, EGF-induced E-cadherin decrease and fibronectin increase were delayed in GnT-III-overexpressing cells. Taken together, these results demonstrated that specific N-glycan alterations were coupled in EMT induced by EGF, which might be contributed to diagnosis and therapy of tumor metastasis. |
| WOS标题词 | Science & Technology ; Life Sciences & Biomedicine |
| 类目[WOS] | Cell Biology |
| 研究领域[WOS] | Cell Biology |
| 关键词[WOS] | CANCER CELLS ; E-CADHERIN ; ACETYLGLUCOSAMINYLTRANSFERASE-III ; BREAST-CANCER ; GROWTH ; ACTIVATION ; METASTASIS ; MICROENVIRONMENT ; GLYCOSYLATION ; MORPHOGENESIS |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000393312200002 |
| 源URL | [http://ir.ipe.ac.cn/handle/122111/21925]  |
| 专题 | 过程工程研究所_研究所（批量导入） |
| 作者单位 | 1.Dalian Ocean Univ, Coll Fisheries & Life Sci, Dalian 116023, Peoples R China 2.Tohoku Med & Pharmaceut Univ, Inst Mol Biomembrane & Glycobiol, Div Regulatory Glycobiol, Sendai, Miyagi 9818558, Japan 3.Dalian Elite Analyt Instruments Co Ltd, Dalian 116023, Peoples R China 4.Chinese Acad Sci, Inst Proc Engn, Beijing 100190, Peoples R China |
| 推荐引用方式 GB/T 7714 | Xu, Qingsong,Qu, Chen,Wang, Wenjing,et al. Specific N-glycan alterations are coupled in epithelial-mesenchymal transition induced by EGF in GE11 epithelial cells[J]. CELL BIOLOGY INTERNATIONAL,2017,41(2):124-133. |
| APA | Xu, Qingsong,Qu, Chen,Wang, Wenjing,Gu, Jianguo,Du, Yuguang,&Song, Linsheng.(2017).Specific N-glycan alterations are coupled in epithelial-mesenchymal transition induced by EGF in GE11 epithelial cells.CELL BIOLOGY INTERNATIONAL,41(2),124-133. |
| MLA | Xu, Qingsong,et al."Specific N-glycan alterations are coupled in epithelial-mesenchymal transition induced by EGF in GE11 epithelial cells".CELL BIOLOGY INTERNATIONAL 41.2(2017):124-133. |
入库方式： OAI收割
来源：过程工程研究所
浏览0
下载0
收藏0
其他版本
除非特别说明，本系统中所有内容都受版权保护，并保留所有权利。