beta 1 integrin signaling in asymmetric migration of keratinocytes under mechanical stretch in a co-cultured wound repair model
文献类型：期刊论文
| 作者 | Lu DY; Li Z; Gao YX; Luo CH; Zhang F; Zheng L; Wang JW; Sun SJ; Long M(龙勉); Gao YX(高宇欣)
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| 刊名 | BIOMEDICAL ENGINEERING ONLINE
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| 出版日期 | 2016 |
| 卷号 | 15页码:130 |
| 通讯作者邮箱 | mlong@imech.ac.cn |
| ISSN号 | 1475-925X |
| 产权排序 | [Lu, Dongyuan; Li, Zhan; Gao, Yuxin; Luo, Chunhua; Zhang, Fan; Zheng, Lu; Wang, Jiawen; Sun, Shujin; Long, Mian] Chinese Acad Sci, Inst Mech, Ctr Biomech & Bioengn, Beijing 100190, Peoples R China; [Lu, Dongyuan; Li, Zhan; Gao, Yuxin; Luo, Chunhua; Zhang, Fan; Zheng, Lu; Wang, Jiawen; Sun, Shujin; Long, Mian] Chinese Acad Sci, Inst Mech, Natl Micrograv Lab, Key Lab Micrograv, Beijing 100190, Peoples R China; [Lu, Dongyuan; Li, Zhan; Gao, Yuxin; Luo, Chunhua; Zhang, Fan; Zheng, Lu; Wang, Jiawen; Sun, Shujin; Long, Mian] Chinese Acad Sci, Inst Mech, Beijing Key Lab Engn Construct & Mechanobiol, Beijing 100190, Peoples R China |
| 通讯作者 | Long, M (reprint author), Chinese Acad Sci, Inst Mech, Ctr Biomech & Bioengn, Beijing 100190, Peoples R China. |
| 中文摘要 | Background: Keratinocyte (KC) migration in re-epithelization is crucial in repairing injured skin. But the mechanisms of how mechanical stimuli regulate the migration of keratinocytes have been poorly understood. Methods: Human immortalized keratinocyte HaCaT cells were co-cultured with skin fibroblasts on PDMS membranes and transferred to the static stretch device developed in-house for additional 6 day culture under mechanical stretch to mimic surface tension in skin. To detect the expression of proteins on different position at different time points and the effect of beta 1 integrin mechanotransduction on HaCaT migration, Immunofluorescence, Reverse transcription-polymerase chain reaction, Flow cytometry, Western blotting assays were applied. Results: Mechanical receptor of beta 1 integrin that recognizes its ligand of collagen I was found to be strongly associated with migration of HaCaT cells since the knockdown of beta 1 integrin via RNA silence eliminated the key protein expression dynamically. Here the expression of vinculin was lower but that of Cdc42 was higher for the cells at outward edge than those at inward edge, respectively, supporting that the migration capability of keratinocytes is inversely correlated with the formation of focal adhesion complexes but positively related to the lamellipodia formation. This asymmetric expression feature was further confirmed by high or low expression of PI3K for outward-or inward-migrating cells. And ERK1/ 2 phosphorylation was up-regulated by mechanical stretch. Conclusion: We reported here, a novel mechanotransduction signaling pathways were beta 1 integrin-dependent pattern of keratinocytes migration under static stretch in an in vitro co-culture model. These results provided an insight into underlying molecular mechanisms of keratinocyte migration under mechanical stimuli. |
| 分类号 | Q3 |
| 类目[WOS] | Engineering, Biomedical |
| 研究领域[WOS] | Engineering |
| 关键词[WOS] | Keratinocyte ; Mechanical stretch ; Fibroblast ; beta 1 integrin ; Mechanotransduction |
| 收录类别 | SCI ; EI |
| 原文出处 | http://dx.doi.org/10.1186/s12938-016-0263-1 |
| 语种 | 英语 |
| WOS记录号 | WOS:000392424700006 |
| 源URL | [http://dspace.imech.ac.cn/handle/311007/60090]  |
| 专题 | 力学研究所_国家微重力实验室 |
| 推荐引用方式 GB/T 7714 | Lu DY,Li Z,Gao YX,et al. beta 1 integrin signaling in asymmetric migration of keratinocytes under mechanical stretch in a co-cultured wound repair model[J]. BIOMEDICAL ENGINEERING ONLINE,2016,15:130. |
| APA | Lu DY.,Li Z.,Gao YX.,Luo CH.,Zhang F.,...&Long M.(2016).beta 1 integrin signaling in asymmetric migration of keratinocytes under mechanical stretch in a co-cultured wound repair model.BIOMEDICAL ENGINEERING ONLINE,15,130. |
| MLA | Lu DY,et al."beta 1 integrin signaling in asymmetric migration of keratinocytes under mechanical stretch in a co-cultured wound repair model".BIOMEDICAL ENGINEERING ONLINE 15(2016):130. |
入库方式： OAI收割
来源：力学研究所
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