Dynamic contributions of P- and E-selectins to beta(2)-integrin-induced neutrophil transmigration
文献类型：期刊论文
| 作者 | Gong YX; Zhang Y; Feng SL; Liu XF; Lv SQ(吕守琴); Long M(龙勉); Zhang Y(章燕) ; Long M(龙勉); Zhang Y(章燕); Zhang Y(章燕)
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| 刊名 | FASEB JOURNAL
 |
| 出版日期 | 2017 |
| 卷号 | 31期号:1页码:212-+ |
| 通讯作者邮箱 | mlong@imech.ac.cn |
| ISSN号 | 0892-6638 |
| 产权排序 | [Gong, Yixin; Zhang, Yan; Feng, Shiliang; Liu, Xiaofeng; Lu, Shouqin; Long, Mian] Chinese Acad Sci, Ctr Biomech & Bioengn, 15 North 4th Ring Rd, Beijing 100190, Peoples R China; [Gong, Yixin; Zhang, Yan; Feng, Shiliang; Liu, Xiaofeng; Lu, Shouqin; Long, Mian] Chinese Acad Sci, Key Lab Micrograv, Natl Micrograv Lab, Beijing, Peoples R China; [Gong, Yixin; Zhang, Yan; Feng, Shiliang; Liu, Xiaofeng; Lu, Shouqin; Long, Mian] Chinese Acad Sci, Inst Mech, Beijing Key Lab Engn Construct & Mechanobiol, Beijing, Peoples R China; [Zhang, Yan; Lu, Shouqin; Long, Mian] Univ Chinese Acad Sci, Sch Engn Sci, Beijing, Peoples R China |
| 通讯作者 | Long, M (reprint author), Chinese Acad Sci, Ctr Biomech & Bioengn, 15 North 4th Ring Rd, Beijing 100190, Peoples R China. ; Long, M (reprint author), Chinese Acad Sci, Inst Mech, Key Lab Micrograv, Natl Micrograv Lab, 15 North 4th Ring Rd, Beijing 100190, Peoples R China. |
| 中文摘要 | Leukocyte transendothelial migrationis a key step in their recruitment to sites of inflammation. However, synergic regulation of endothelium-expressed selectins on leukocyte transmigration remains unclear. In this study, an in vitro model was developed to investigate the dynamic contributions of P- and E-selectin to polymorphonuclear neutrophil (PMN) transmigration under static conditions. Human umbilical vein endothelial cells (HUVECs) were treated with LPS for 4 or 12 h to induce different expression of selectins and intercellular adhesion molecule (ICAM)-1. PMN transmigration was increased significantly by LPS stimulation, which was higher on 4-h than on 12-h LPS treated HUVECs. Blocking and competitive tests indicated that P-selectin engages PSGL-1 to activate beta(2)-integrin and initiate PMN transmigration within the first 15 min, whereas E-selectin engages CD44 to influence PMN transmigration after 15 min. P-and E-selectin-induced beta(2)-integrin activation is likely conducted through the spleen tyrosine kinase signaling pathway. Complicated complementary and competitive mechanisms are involved in the interaction of P-/E-selectins and their ligands to promote PMN transmigration. These results provide direct evidence of the distinct and dynamic contribution of P- and E-selectins inmediating PMN transmigration and give new insight into PMN interaction with the vessel wall.-Gong, Y., Zhang, Y., Feng, S., Liu, X., Lu, S., Long, M. Dynamic contributions of P-and E-selectins to beta(2)-integrin-induced neutrophil transmigration. |
| 分类号 | 一类 |
| 类目[WOS] | Biochemistry & Molecular Biology ; Biology ; Cell Biology |
| 研究领域[WOS] | Biochemistry & Molecular Biology ; Life Sciences & Biomedicine - Other Topics ; Cell Biology |
| 关键词[WOS] | PSGL-1 ; CD44 ; synergic mechanisms |
| 收录类别 | SCI |
| 原文出处 | http://dx.doi.org/10.1096/fj.201600398RRR |
| 语种 | 英语 |
| WOS记录号 | WOS:000392177600022 |
| 源URL | [http://dspace.imech.ac.cn/handle/311007/60110]  |
| 专题 | 力学研究所_国家微重力实验室 |
| 推荐引用方式 GB/T 7714 | Gong YX,Zhang Y,Feng SL,et al. Dynamic contributions of P- and E-selectins to beta(2)-integrin-induced neutrophil transmigration[J]. FASEB JOURNAL,2017,31(1):212-+. |
| APA | Gong YX.,Zhang Y.,Feng SL.,Liu XF.,吕守琴.,...&Zhang Y.(2017).Dynamic contributions of P- and E-selectins to beta(2)-integrin-induced neutrophil transmigration.FASEB JOURNAL,31(1),212-+. |
| MLA | Gong YX,et al."Dynamic contributions of P- and E-selectins to beta(2)-integrin-induced neutrophil transmigration".FASEB JOURNAL 31.1(2017):212-+. |
入库方式： OAI收割
来源：力学研究所
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