A horsefly saliva antigen 5-like protein containing RTS motif is an angiogenesis inhibitor
文献类型：期刊论文
| 作者 | Ma DY1,4; Gao L1,4; Xu XQ1,4; Lai R[*]1,3; An S2,4; Song YZ1,4; Wu J1,4 |
| 刊名 | Toxicon
 |
| 出版日期 | 2010 |
| 卷号 | 55期号:X页码:45-51 |
| 关键词 | Horsefly Salivary glands Disintegrin Angiogenesis inhibitor Tabanus yao |
| 通讯作者 | rlai@mail.kiz.ac.cn |
| 英文摘要 | The Ag5 proteins are the most abundant and immunogenic proteins in the venom secretory ducts of stinging insects. An antigen 5-likeprotein (named tabRTS) composed of 221 amino acid residues was purified and characterized from the salivary glands of the horsefly, Tabanus yao (Diptera, Tabanidae). Its cDNA was cloned from the cDNA library of the horsefly's salivary gland. TabRTS containing the SCP domain (Sc7 family of extracellular protein domain) was found in insect antigen 5 proteins. More interestingly, there is an Arg-Thr-Ser (RTS) disintegrin motif at the C-terminus of tabRTS. The RTS motif is positioned in a loop bracketed by cysteine residues as those found in RTS-disintegrins of Crotalidae and Viperidae snake venoms, which act as angiogenesis inhibitors. Endothelial Cell Tube formation assay in vitro and chicken chorioallantoic membrane (CAM) angiogenesis assay in vivo were performed as to investigate the effect of tabRTS on angiogenesis. It was found that tabRTS could significantly inhibit angiogenesis in vitro and in vivo. Anti-alpha(1)beta(1) monoclonal antibody could dose-dependently inhibit the anti-angiogenic activity of tabRTS. This result indicated that tabRTS possibly targets the alpha(1)beta(1) integrin to exert the anti-angiogenic activity as snake venom RTS-/KTS-disintegrins do. The current work revealed the first angiogenesis inhibitor protein containing RTS motif from invertebrates, a possible novel type ofRTS-disintegrin. |
| 收录类别 | SCI |
| 资助信息 | This work was supported by the Chinese National Natural Science Foundation (30830021), Chinese Academy of Sciences (KSCXZ-YW-R-088, KSCX2-YW-G-024), and the Ministry of Science (2007AA02Z192, 2007AA100602). |
| 语种 | 英语 |
| 源URL | [http://159.226.149.26:8080/handle/152453/11012]  |
| 专题 | 昆明动物研究所_动物模型与人类重大疾病机理重点实验室 昆明动物研究所_动物毒素室 |
| 作者单位 | 1.Biotoxin Units of Key Laboratory of Animal Models and Human Disease Mechanisms, Kunming Institute of Zoology, Chinese Academy of Sciences, 32# Jiaochangdonglu, Kunming 650223, Yunnan, China 2.School of Life Sciences, University of Science and Technology of China, Hefei 230026, Anhui, China 3.Key Laboratory of Microbiological Engineering of Agricultural Environment, Ministry of Agriculture, Life Sciences College of Nanjing Agricultural University, Nanjing, Jiangsu 210095, China 4.Graduate School of the Chinese Academy of Sciences, Beijing 100009, China |
| 推荐引用方式 GB/T 7714 | Ma DY,Gao L,Xu XQ,et al. A horsefly saliva antigen 5-like protein containing RTS motif is an angiogenesis inhibitor[J]. Toxicon,2010,55(X):45-51. |
| APA | Ma DY.,Gao L.,Xu XQ.,Lai R[*].,An S.,...&Wu J.(2010).A horsefly saliva antigen 5-like protein containing RTS motif is an angiogenesis inhibitor.Toxicon,55(X),45-51. |
| MLA | Ma DY,et al."A horsefly saliva antigen 5-like protein containing RTS motif is an angiogenesis inhibitor".Toxicon 55.X(2010):45-51. |
入库方式： OAI收割
来源：昆明动物研究所
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