Genetic diversity and matrilineal structure in Chinese tree shrews inhabiting Kunming, China
文献类型：期刊论文
| 作者 | Chen SY1; Xu L1,3; Lv LB2; Yao YG[*]1 |
| 刊名 | 动物学研究
 |
| 出版日期 | 2011 |
| 卷号 | 32期号:1页码:17-23 |
| 关键词 | Chinese tree shrews mtDNA Control region Cytochorme b Genetic diversity |
| 其他题名 | 昆明城郊中国树鼩群体线粒体DNA遗传多样性 |
| 通讯作者 | ygyaozh@gmail.com |
| 合作状况 | 其它 |
| 中文摘要 | 由于树鼩是灵长类动物的近亲,且具有体型小、繁殖周期短、饲养管理成本低等优点,长期以来被认为有望替代灵长类动物用于人类疾病的动物模型研究。然而,目前对树鼩的群体遗传结构还知之甚少,这极大地限制了其在疾病动物模型研究的应用,也是其品系资源创制的瓶颈。本研究通过分析80只采自于云南省昆明周边地区的野生树鼩(Tupaia belangeri chinensis)线粒体DNA(mtDNA)多态性,结合国外报道的2个树鼩(Tupaia belangeri)序列比较后发现,在604bp的mtDNA控制区片段中共检测到29个核苷酸替代变异,这些变异共界定了13种单倍型,表现较高的群体遗传多样度。另外,昆明地区的树鼩与国外报道的2个树鼩间存在较大的遗传分化,mtDNA控制区单倍型之间的核苷酸替换数大于18个,远高于昆明地区树鼩群体内部不同单倍型之间的差异。选择含有代表性的mtDNA控制区单倍型的17个昆明地区树鼩个体进一步测定了细胞色素b基因片段(1134bp),结合前人报道的数据分析,结果进一步支持mtDNA控制区数据反映的遗传格局及揭示的昆明地区树鼩与国外报道树鼩之间的明显差异。本研究结果提示,昆明地区树鼩与国外树鼩之间存... |
| 英文摘要 | Due to their special phylogenetic position in the Euarchontoglires and close affinity to primates, tree shrews have been proposed as an alternative experimental animal to primates in biomedical research. However, the population genetic structure of tree shrews has largely remained unknown and this has hindered the development of tree shrew breeding and selection. Here we sampled 80 Chinese tree shrews (Tupaia belangeri chinensis) in Kunming, China, and analyzed partial mtDNA control region sequence variation. Based on our samples and two published sequences from northern tree shrews (T. belangeri), we identified 29 substitutions in the mtDNA control region fragment (~ 604 bp) across 82 individuals and defined 13 haplotypes. Seventeen samples were selected for sequencing of the cytochrome b (Cyt b; 1134 bp) gene based on control region sequence variation and were analyzed in combination with 34 published sequences to solidify the phylogenetic pattern obtained from control region data. Overall, tree shrews from Kunming have high genetic diversity and present a remarkable long genetic distance to the two reported northern tree shrews outside China. Our results provide some caution when using tree shrews to establish animal models because of this apparent genetic difference. In addition, the high genetic diversity of Chinese tree shrews inhabiting Kunming suggests that systematic genetic investigations should be conducted before establishing an inbred strain for medical and biological research |
| 收录类别 | 其他 |
| 资助信息 | Supported by grants from Yunnan Province (2009CI119);the Chinese Academy of Sciences (KSCX2-EW-R-11 and KSCX2-EW-J-23);the Key Laboratory of Animal Models and Human Disease Mechanisms of the Chinese Academy of Sciences & Yunnan Province. |
| 语种 | 英语 |
| 公开日期 | 2011-08-23 |
| 源URL | [http://159.226.149.42:8088/handle/353002/6684]  |
| 专题 | 昆明动物研究所_重大疾病机理的遗传学 昆明动物研究所_动物模型与人类重大疾病机理重点实验室 昆明动物研究所_中国科学院昆明灵长类研究中心 |
| 作者单位 | 1.Key Laboratory of Animal Models and Human Disease Mechanisms of the Chinese Academy of Sciences & Yunnan Province, Kunming Institute of Zoology, Kunming Yunnan 650223, China 2.Experimental Animal Core Facility & Kunming Primate Research Center, Kunming Institute of Zoology, the Chinese Academy of Sciences, Kunming Yunnan 650223, China 3.Graduate School of the Chinese Academy of Sciences, Beijing 100049, China |
| 推荐引用方式 GB/T 7714 | Chen SY,Xu L,Lv LB,et al. Genetic diversity and matrilineal structure in Chinese tree shrews inhabiting Kunming, China[J]. 动物学研究,2011,32(1):17-23. |
| APA | Chen SY,Xu L,Lv LB,&Yao YG[*].(2011).Genetic diversity and matrilineal structure in Chinese tree shrews inhabiting Kunming, China.动物学研究,32(1),17-23. |
| MLA | Chen SY,et al."Genetic diversity and matrilineal structure in Chinese tree shrews inhabiting Kunming, China".动物学研究 32.1(2011):17-23. |
入库方式： OAI收割
来源：昆明动物研究所
浏览0
下载0
收藏0
其他版本
除非特别说明，本系统中所有内容都受版权保护，并保留所有权利。