PTEN/PIK3CA genes are frequently mutated in spontaneous and medroxyprogesterone acetate-accelerated 7,12-dimethylbenz(a)anthracene-induced mammary tumours of tree shrews
文献类型：期刊论文
| 作者 | Xia HJ1; He BL2; Wang CY1,3,4; Zhang HL1; Ge GZ1,5; Zhang YX1; Lv LB1; Jiao JL2; Chen CS[*]1 |
| 刊名 | EUROPEAN JOURNAL OF CANCER
 |
| 出版日期 | 2014 |
| 卷号 | 50期号:18页码:3230-3242 |
| 关键词 | Breast cancer Tree shrew Animal model PTEN PIK3CA Gene mutation |
| 通讯作者 | chenc@mail.kiz.ac.cn |
| 合作状况 | 其它 |
| 英文摘要 | Tree shrew has increasingly become an attractive experimental animal model for human diseases, particularly for breast cancer due to spontaneous breast tumours and their close relationship to primates and by extension to humans. However, neither normal mammary glands nor breast tumours have been well characterised in the Chinese tree shrew (Tupaia belangeri chinensis). In this study, normal mammary glands from four different developmental stages and 18 spontaneous breast tumours were analysed. Haematoxylin and eosin (H&E) staining and immunohistochemistry (IHC) showed that normal mammary gland morphology and structures of tree shrews were quite similar to those found in humans. Spontaneous breast tumours of tree shrews were identified as being intraductal papilloma, papillary carcinoma, and invasive ductal carcinoma with or without lung metastasis. To further analyse breast cancer tumours among tree shrews, 40 3-4 month-old female tree shrews were orally administrated 20 mg 7,12-dimethylbenz(a)anthracene (DMBA) or peanut oil thrice, and then, 15 of these DMBA administrated tree shrews were implanted with medroxyprogesterone acetate (MPA) pellets. DMBA was shown to induce breast tumours (12%) while the addition of MPA increased the tumour incidence (50%). Of these, three induced breast tumours were intraductal papillary carcinomas and one was invasive ductal carcinoma (IDC). The PTEN/PIK3CA (phosphatase and tensin homologue/phosphatidylinositol-4,5-bisphosphate 3-kinase catalytic subunit alpha), but not TP53 and GATA3, genes are frequently mutated in breast tumours, and the PTEN/PIK3CA gene mutation status correlated with the expression of pAKT in tree shrew breast tumours. These results suggest that tree shrews may be a promising animal model for a subset of human breast cancers with PTEN/PIK3CA gene mutations. |
| 收录类别 | SCI |
| 资助信息 | This work was partially supported by the 863 Project (2012AA021801, 2012AA022402) from Ministry of Sci- ence and Technology of China, National Natural Science Foundation of China (81202110, 81120108019, U1132605, and 81325016), and grants from Chinese AcademyofSciences(KSCX2-EW-J-23andthe‘Western Light’ Talents Training Program (to HJ Xia)). |
| 语种 | 英语 |
| 源URL | [http://159.226.149.26:8080/handle/152453/9234]  |
| 专题 | 昆明动物研究所_中国科学院昆明灵长类研究中心 昆明动物研究所_动物模型与人类重大疾病机理重点实验室 昆明动物研究所_肿瘤生物学 |
| 作者单位 | 1.Key Laboratory of Animal Models and Human Disease Mechanisms of Chinese Academy of Sciences and Yunnan Province, Kunming Institute of Zoology, Chinese Academy of Sciences, Kunming, Yunnan 650223, China 2.Department of Laboratory Animal Science, Kunming Medical University, Kunming, Yunnan 650500, China 3.Department of Pathology, First Affiliated Hospital of Kunming Medical University, Kunming, Yunnan 650032, China 4.Kunming College of Life Science, University of Chinese Academy of Sciences, Kunming, Yunnan 650204, China 5.Faculty of Life Science and Technology, Kunming University of Science and Technology, Kunming, Yunnan 650500, China |
| 推荐引用方式 GB/T 7714 | Xia HJ,He BL,Wang CY,et al. PTEN/PIK3CA genes are frequently mutated in spontaneous and medroxyprogesterone acetate-accelerated 7,12-dimethylbenz(a)anthracene-induced mammary tumours of tree shrews[J]. EUROPEAN JOURNAL OF CANCER,2014,50(18):3230-3242. |
| APA | Xia HJ.,He BL.,Wang CY.,Zhang HL.,Ge GZ.,...&Chen CS[*].(2014).PTEN/PIK3CA genes are frequently mutated in spontaneous and medroxyprogesterone acetate-accelerated 7,12-dimethylbenz(a)anthracene-induced mammary tumours of tree shrews.EUROPEAN JOURNAL OF CANCER,50(18),3230-3242. |
| MLA | Xia HJ,et al."PTEN/PIK3CA genes are frequently mutated in spontaneous and medroxyprogesterone acetate-accelerated 7,12-dimethylbenz(a)anthracene-induced mammary tumours of tree shrews".EUROPEAN JOURNAL OF CANCER 50.18(2014):3230-3242. |
入库方式： OAI收割
来源：昆明动物研究所
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