大肠杆菌mRNA 编码区长度、形成二级结构倾向与密码子偏好性的关系
文献类型：期刊论文
| 作者 | 王侃1; 刘次全[*]1,2,3,4; 曹槐1; 陈雪峰1 |
| 刊名 | 微生物学报
 |
| 出版日期 | 2006 |
| 卷号 | 46期号:6页码:895-899 |
| 关键词 | 大肠杆菌 密码子偏好性 mRNA编码区 mRNA二级结构 翻译效率 |
| 其他题名 | Insight into relationship among mRNA coding region length, folding tendency and codon usage bias in Escherichia coli |
| 通讯作者 | liucq@ynu.edu.cn |
| 合作状况 | 其它 |
| 中文摘要 | 从GenBank获得大肠杆菌K-12MG1655株的全基因组序列,计算了与基因密码子偏好性相关的多个参数(Nc、CAI、GC、GC3s),对其mRNA编码区长度、形成二级结构倾向与密码子偏好性之间的关系进行了统计学分析,发现虽然翻译效率(包括翻译速度和翻译精度)是制约大肠杆菌高表达基因的密码子偏好性的主要因素,同时,mRNA编码区长度及其形成二级结构的倾向也是形成这种偏好性的不可忽略的原因,而且对偏好性有一定程度的削弱。另外对mRNA编码区形成二级结构倾向的生物学意义进行了讨论分析。 |
| 英文摘要 | Escherichia coli has been regarded as a model organism in the study of codon usage bias. Most studies in this organism regarding this topic have focused their mind on translation efficiency ( translation speed and translation precision) . However , some genes with low codon usage bias and high expression can not be explained by translation efficiency. And some evidence of local RNA secondary structure in coding region of Escherichia coli genes have been given. All of these need explanation from a new point of view. The genomic sequence of Escherichia coli K-12 MG1655 was obtained from GenBank. Several parameter (Nc ,CAI ,GC ,GC3s) of 4199 genes on codon usage bias were computed. Folding tendency of mRNA coding region was represented by its average energy of 50 nucleotides. And then , relationship among mRNA coding region length , folding tendency and codon usage bias in Escherichia coli were studied. It is argued that though translation efficiency is a primary cause that shapes the codon usage bias of highly expressed genes in Escherichia coli , mRNA coding region length and folding tendency are also unneglectable factor for codon usage bias and weakening of bias. In addition , biological significance of folding tendency in mRNA coding regions was discussed. |
| 资助信息 | 国家自然科学基金重大研究计划项目(90208018,90303018);; 国家自然科学基金数学天元基金重点项目(A0324101)~~ |
| 收录类别 | 其他 |
| 语种 | 中文 |
| 公开日期 | 2010-08-09 |
| 源URL | [http://159.226.149.42:8088/handle/152453/1120]  |
| 专题 | 昆明动物研究所_其他 |
| 作者单位 | 1.云南大学现代生物学中心 昆明650091 2.中国科学院昆明动物研究所 昆明650223 3.北京大学理论生物学中心 北京100871 4.大连理工大学高科技研究院;大连116024 |
| 推荐引用方式 GB/T 7714 | 王侃,刘次全[*],曹槐,等. 大肠杆菌mRNA 编码区长度、形成二级结构倾向与密码子偏好性的关系[J]. 微生物学报,2006,46(6):895-899. |
| APA | 王侃,刘次全[*],曹槐,&陈雪峰.(2006).大肠杆菌mRNA 编码区长度、形成二级结构倾向与密码子偏好性的关系.微生物学报,46(6),895-899. |
| MLA | 王侃,et al."大肠杆菌mRNA 编码区长度、形成二级结构倾向与密码子偏好性的关系".微生物学报 46.6(2006):895-899. |
入库方式： OAI收割
来源：昆明动物研究所
浏览0
下载0
收藏0
其他版本
除非特别说明，本系统中所有内容都受版权保护，并保留所有权利。