HCCS1 overexpression induces apoptosis via cathepsin D and intracellular calcium, and HCCS1 disruption in mice causes placental abnormality
文献类型：期刊论文
| 作者 | Gan Y1; Zhao X2; Hu J1; Wang ZG[*]2; Zhao XD[*]1 |
| 刊名 | Cell Death and Differentiation
 |
| 出版日期 | 2008 |
| 卷号 | 15期号:9页码:1481-1490 |
| 关键词 | hepatocellular carcinoma suppressor 1 calcium cathepsin D labyrinth abnormality |
| 通讯作者 | xtzhao@online.sh.cn ; zhugangw@shsmu.edu.cn |
| 英文摘要 | Hepatocellular carcinoma suppressor 1 (HCCS1) was discovered as a novel tumor suppressor gene. We recently observed that adenovirus-mediated gene transfer of HCCS1 leads to cytotoxicity to human hepatocarcinoma cells. Here, we have demonstrated that adenovirus-mediated overexpression of HCCS1 induces apoptosis in hepatocarcinoma cells and have further characterized the apoptotic cascade. The results showed that lysosomal cathepsin D is released into the cytosol in response to HCCS1 overexpression and consequently triggers Bax insertion into the mitochondrial membrane, which leads to the release of cytochrome c. In addition, HCCS1 overexpression can induce an increase in intracellular free Ca(2+) concentration, which also results in cytochrome c release. The released cytochrome c activates downstream caspases, leading to the occurrence of the late stages of apoptosis. Moreover, we demonstrated that the disruption of HCCS1 in mice leads to embryonic lethality, accompanied by abnormal labyrinth architecture resulting from the excessive proliferation of trophoblast cells in the placenta. These results suggest that HCCS1 plays a role in apoptosis regulation and development. |
| 收录类别 | SCI |
| 资助信息 | This work was supported by grants from the Key Programs of the National Natural Science Foundation of China (No. 30330350) and the Shanghai Committee of Science and Technology (No. 04XD14015). |
| 语种 | 英语 |
| 公开日期 | 2014-07-07 |
| 源URL | [http://159.226.149.42:8088/handle/152453/7941]  |
| 专题 | 昆明动物研究所_科研共享资源 |
| 作者单位 | 1.Laboratory for Cellular and Molecular Immunology, Shanghai Cancer Institute, Shanghai 200032, P.R. China 2.Shanghai Research Center for Model Organisms, Shanghai 201210, P.R. China |
| 推荐引用方式 GB/T 7714 | Gan Y,Zhao X,Hu J,et al. HCCS1 overexpression induces apoptosis via cathepsin D and intracellular calcium, and HCCS1 disruption in mice causes placental abnormality[J]. Cell Death and Differentiation,2008,15(9):1481-1490. |
| APA | Gan Y,Zhao X,Hu J,Wang ZG[*],&Zhao XD[*].(2008).HCCS1 overexpression induces apoptosis via cathepsin D and intracellular calcium, and HCCS1 disruption in mice causes placental abnormality.Cell Death and Differentiation,15(9),1481-1490. |
| MLA | Gan Y,et al."HCCS1 overexpression induces apoptosis via cathepsin D and intracellular calcium, and HCCS1 disruption in mice causes placental abnormality".Cell Death and Differentiation 15.9(2008):1481-1490. |
入库方式： OAI收割
来源：昆明动物研究所
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