中国科学院机构知识库网格
Chinese Academy of Sciences Institutional Repositories Grid
PCR-based approaches for identification of multi-copy transgene integration sites in mouse genome

文献类型:期刊论文

作者Zhao XD1,2; Dang SY2; Liang B1; Lei X2; Chen Z1; Wang L3; Yan LZ1; Sun HT1; Fei JL1; Fei J1
刊名CHINESE SCIENCE BULLETIN
出版日期2006
卷号51期号:8页码:2231-2235
关键词trangene integration site insertional mutation PCR
通讯作者zhugangw@shsmu.edu.cn
英文摘要Generation of transgenic mice by DNA microinjection has become one of the most important technologies in studying gene function in vivo. Random integration of transgene often results in insertional mutation which may complicate phenotype analysis of transgenic mice and/or create a good opportunity to study the function of endogenous gene. However, the utilization of these potentially valuable resources is hampered due to lack of efficient approaches for rapid identification of multi-copy transgene integration sites. Here we propose two PCR-based approaches to identifying transgene/genome junction sequences. The efficiency of these two strategies was tested in 9 independent transgenic mouse lines. Among them, the transgene in 8 mouse lines was clearly localized to certain chromosome regions. These rapid and efficient approaches will greatly facilitate the study of insertional mutation due to transgene integration.
收录类别SCI
资助信息This work was supported by the grants from Science & Technology Committee of Shanghai Mu- nicipality (Grant No. 03DZ14022), the National High Tech- nology Research and Development Program of China (Grant No. 2001AA216081), and the National Natural Science Foundation of China for Outstanding Young Scientists (Grant No. 39925023) .
语种英语
公开日期2014-07-07
源URL[http://159.226.149.42:8088/handle/152453/7944]  
专题昆明动物研究所_科研共享资源
作者单位1.Shanghai Research Center for Model Organisms, Shanghai 201203, China
2.Laboratory of Genetic Engineering, Department of Medical Genetics, Institute of Health Science jointly-funded by Shanghai Institutes for Biological Sciences of Chinese Academy of Sciences and Shanghai Second Medical University, Shanghai 200025, China
3.State Key Laboratory of Medical Genomics, Rui-Jin Hospital Affili- ated to Shanghai Second Medical University, Shanghai 200025, China
推荐引用方式
GB/T 7714
Zhao XD,Dang SY,Liang B,et al. PCR-based approaches for identification of multi-copy transgene integration sites in mouse genome[J]. CHINESE SCIENCE BULLETIN,2006,51(8):2231-2235.
APA Zhao XD.,Dang SY.,Liang B.,Lei X.,Chen Z.,...&Wang ZG[*].(2006).PCR-based approaches for identification of multi-copy transgene integration sites in mouse genome.CHINESE SCIENCE BULLETIN,51(8),2231-2235.
MLA Zhao XD,et al."PCR-based approaches for identification of multi-copy transgene integration sites in mouse genome".CHINESE SCIENCE BULLETIN 51.8(2006):2231-2235.

入库方式: OAI收割

来源:昆明动物研究所

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