Wnt pathway is involved in pleomorphic adenomas induced by overexpression of PLAG1 in transgenic mice
文献类型：期刊论文
| 作者 | Zhao XD1,2; Ren WH1; Yang WJ3; Wang Y2; Kong H2; Wang L2,4; Yan LZ2; Xu GJ2; Fei J2; Zhang CP3 |
| 刊名 | INTERNATIONAL JOURNAL OF CANCER
 |
| 出版日期 | 2006 |
| 卷号 | 118期号:3页码:643-648 |
| 关键词 | pleomorphic adenoma gene 1 transgenic mice beta-catenin pleomorphic adenomas |
| 通讯作者 | zhugangw@shsmu.edu.cn |
| 合作状况 | 其它 |
| 英文摘要 | Pleomorphic adenoma gene 1 (PLAG1) was found frequently rearranged and activated in human salivary gland pleomorphic adenomas. It encodes a developmentally regulated transcription factor. Ectopic overexpression of PLAG1 has been proposed to play a crucial role in tumorigenesis of salivary gland pleomorphic adenomas. It was reported that PLAG1 can activate the transcription of insulin-like growth factor 2 (IGF2), functioning as a protooncogene. In this report, we show that the salivary gland tumors developed in PLAG1 transgenic mice share major histopathologic features with human pleomorphic adenomas. It was found that beta-catenin, the key component of Wnt signaling pathway, was upregulated at transcriptional level in tumors developed in 3 independent transgenic mouse lines. Immunohistochemical staining revealed that expression of beta-catenin as well as c-myc, downstream of beta-catenin in Wnt signaling pathway, was highly upregulated with overexpression of PLAG1 transgene in tumor and normal transgenic salivary gland tissues. Moreover, we found that PLAG1 can activate the transcription of mouse but not human beta-catenin in the 3T3 cells cotransfected with reporter constructs. Sequence analysis shows there are 4 PLAG1 consensus binding sites in mouse beta-catenin promoter region but not in human. Our findings provide the first in vivo evidence for the oncogenic activity of PLAG1 in pleomorphic adenoma tumorigenesis, reveal a valued animal model for human salivary gland tumors and suggest that Wnt signaling pathway may also contribute to the development of pleomorphic adenomas in transgenic mice. |
| 收录类别 | SCI |
| 资助信息 | the Major State Basic Research Development Program of China; Grant number: 2001CB509901; Grant sponsor: the National High Technology Research and Development Program of China; Grant number: 2001AA216081; Grant sponsor: the National Natural Science Foundation of China for Outstanding Young Scientists; Grant number: 39925023; Grant sponsor: E-Institutes of Shanghai Municipal Education Commission; Grant number: E03003; Grant sponsor: The National Natural Science Foundation of China; Grant number: 30271420. |
| 语种 | 英语 |
| 公开日期 | 2014-07-07 |
| 源URL | [http://159.226.149.42:8088/handle/152453/7949]  |
| 专题 | 昆明动物研究所_科研共享资源 |
| 作者单位 | 1.Laboratory of Genetic Engineering, Department of Medical Genetics, Institute of Health Science, Shanghai Institutes for Biologic Sciences of Chinese Academy of Sciences and Shanghai Second Medical University, Shanghai, China 2.Shanghai Research Center for Model Organisms, Shanghai, China 3.Department of Oral and Maxillofacial Surgery, Ninth People’s Hospital Affiliated to Shanghai Second Medical University, Shanghai, China 4.State Key Laboratory of Medical Genomics, Rui-Jin Hospital Affiliated to Shanghai Second Medical University, Shanghai, China |
| 推荐引用方式 GB/T 7714 | Zhao XD,Ren WH,Yang WJ,et al. Wnt pathway is involved in pleomorphic adenomas induced by overexpression of PLAG1 in transgenic mice[J]. INTERNATIONAL JOURNAL OF CANCER,2006,118(3):643-648. |
| APA | Zhao XD.,Ren WH.,Yang WJ.,Wang Y.,Kong H.,...&Wang ZG[*].(2006).Wnt pathway is involved in pleomorphic adenomas induced by overexpression of PLAG1 in transgenic mice.INTERNATIONAL JOURNAL OF CANCER,118(3),643-648. |
| MLA | Zhao XD,et al."Wnt pathway is involved in pleomorphic adenomas induced by overexpression of PLAG1 in transgenic mice".INTERNATIONAL JOURNAL OF CANCER 118.3(2006):643-648. |
入库方式： OAI收割
来源：昆明动物研究所
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