环介导等温扩增技术检测旋毛虫的研究
文献类型：期刊论文
| 作者 | 李小红1; 曹建平[*]1; 王芸2; 徐裕信1 |
| 刊名 | 国际医学寄生虫病杂志
 |
| 出版日期 | 2013 |
| 卷号 | 40期号:5页码:262-264 |
| 关键词 | 旋毛虫 环介导等温扩增 检测 |
| 其他题名 | Detection of DNA derived from Trichinella spiralis by loop-mediated isothermal amplification |
| 通讯作者 | caojp@yahoo.com |
| 合作状况 | 其它 |
| 中文摘要 | 目的用环介导等温扩增（loop-mediated isothermal amplification,LAMP）技术检测旋毛虫DNA。方法用酚．氯仿法提取旋毛虫基因组DNA，设计两对扩增旋毛虫18SrRNA基因的LAMP引物．以日本血吸虫DNA作对照．进行LAMP反应。将旋毛虫基因组DNA经梯度稀释，进行LAMP反应，验证该方法的敏感性。产物经SYBRGreenI显色后观察结果，绿色判为阳性，棕色判为阴性。结果LAMP反应结果显示．旋毛虫基因检测管经显色后呈绿色，对照组均呈棕色。LAMP技术可检测到的旋毛虫最低浓度为415fg/μl。结论建立了检测旋毛虫DNA的LAMP技术。 |
| 英文摘要 | Objective To detect Trichinella spiralis DNA by loop-mediated isothermal amplification （LAMP）. Methods DNA was extracted by phenol-chloroform extraction method from TrichineUa larva. Four primers were designed based on 18S rRNA sequence and used for LAMP assay. To evaluate the specificity of the LAMP method, DNA sample derived from Schistosoma japonicum was used as control. For evaluating the sensitivity of LAMP, the genomic DNA derived from T. spira!is was serially diluted and amplified by LAMP. LAMP reaction mixture was stained with SYBR green I dye and the resuh was determined according to the color developed in the tube by the naked eye. Green indicated positive reaction while brown was negative. Results After LAMP reaction,the test tube which used genomic DNA derived from T. spiralis appeared to be green, while control was brown. The sensitivity of LAMP assay in this study reached up to 415 fg/μl. Conclusion LAMP assay was established for the detection of DNA sample of T. spiralis. |
| 收录类别 | 其他 |
| 资助信息 | 上海市卫生局青年科研项目（IPD200602） |
| 语种 | 中文 |
| 公开日期 | 2014-10-27 |
| 源URL | [http://159.226.149.42:8088/handle/152453/8150]  |
| 专题 | 昆明动物研究所_中国科学院昆明灵长类研究中心 |
| 作者单位 | 1.中国疾病预防控制中心寄生虫病预防控制所,WHO疟疾、血吸虫病和丝虫病合作中心,卫生部寄生虫病原与媒介生物学重点实验室,上海200025 2.中国科学院昆明动物研究所,昆明650223 |
| 推荐引用方式 GB/T 7714 | 李小红,曹建平[*],王芸,等. 环介导等温扩增技术检测旋毛虫的研究[J]. 国际医学寄生虫病杂志,2013,40(5):262-264. |
| APA | 李小红,曹建平[*],王芸,&徐裕信.(2013).环介导等温扩增技术检测旋毛虫的研究.国际医学寄生虫病杂志,40(5),262-264. |
| MLA | 李小红,et al."环介导等温扩增技术检测旋毛虫的研究".国际医学寄生虫病杂志 40.5(2013):262-264. |
入库方式： OAI收割
来源：昆明动物研究所
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