An Efficient Protocol for Preparation of Gallic Acid from Terminalia bellirica (Gaertn.) Roxb by Combination of Macroporous Resin and Preparative High-Performance Liquid Chromatography
文献类型：期刊论文
| 作者 | Zou, Denglang1,2,3; Chen, Tao1,2; Chen, Chen1,2; Li, Hongmei1,2,3; Liu, Yongling1,2; Li, Yulin1,2 |
| 刊名 | journal of chromatographic science
 |
| 出版日期 | 2016-08-01 |
| 卷号 | 54期号:7页码:1220-1224 |
| 英文摘要 | in this article, macroporous resin column chromatography and preparative high-performance liquid chromatography were applied for preparation of gallic acid from terminalia bellirica (gaertn.) roxb. in the first step, six kinds of resins were investigated by adsorption and desorption tests and ab-8 macroporous resin was selected for the enrichment of gallic acid. as a result, 20 g of gallic acid at a purity of 71% could be separated from 100 g of crude extract in which the content of gallic acid was 16.7% and the recovery of gallic acid reached 85.0%. in the second step, preparative high-performance liquid chromatography was selected to purify gallic acid. as a result, 640 mg of gallic acid at a purity of 99.1% was obtained from 1 g of sample in 35 min. the results demonstrated that macroporous resin coupled with preparative high-performance liquid chromatography was suitable for preparation of gallic acid from t. bellirica (gaertn.) roxb. |
| WOS标题词 | science & technology ; life sciences & biomedicine ; physical sciences |
| 类目[WOS] | biochemical research methods ; chemistry, analytical |
| 研究领域[WOS] | biochemistry & molecular biology ; chemistry |
| 关键词[WOS] | counter-current chromatography ; adsorption chromatography ; purification ; separation ; antioxidant ; belerica ; fruits ; extraction ; flavonoids ; enrichment |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000389138700021 |
| 源URL | [http://ir.nwipb.ac.cn/handle/363003/6655]  |
| 专题 | 西北高原生物研究所_中国科学院西北高原生物研究所 |
| 作者单位 | 1.Chinese Acad Sci, Northwest Inst Plateau Biol, Key Lab Adaptat & Evolut Plateau Biota, Xining 810008, Peoples R China 2.Chinese Acad Sci, Northwest Inst Plateau Biol, Key Lab Tibetan Med Res, Xining 810008, Peoples R China 3.Chinese Acad Sci, Grad Sch, Beijing 100049, Peoples R China |
| 推荐引用方式 GB/T 7714 | Zou, Denglang,Chen, Tao,Chen, Chen,et al. An Efficient Protocol for Preparation of Gallic Acid from Terminalia bellirica (Gaertn.) Roxb by Combination of Macroporous Resin and Preparative High-Performance Liquid Chromatography[J]. journal of chromatographic science,2016,54(7):1220-1224. |
| APA | Zou, Denglang,Chen, Tao,Chen, Chen,Li, Hongmei,Liu, Yongling,&Li, Yulin.(2016).An Efficient Protocol for Preparation of Gallic Acid from Terminalia bellirica (Gaertn.) Roxb by Combination of Macroporous Resin and Preparative High-Performance Liquid Chromatography.journal of chromatographic science,54(7),1220-1224. |
| MLA | Zou, Denglang,et al."An Efficient Protocol for Preparation of Gallic Acid from Terminalia bellirica (Gaertn.) Roxb by Combination of Macroporous Resin and Preparative High-Performance Liquid Chromatography".journal of chromatographic science 54.7(2016):1220-1224. |
入库方式： OAI收割
来源：西北高原生物研究所
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