Determination of Triterpene Acids from 37 Different Varieties of Raspberry using Pre-column Derivatization and HPLC Fluorescence Detection
文献类型：期刊论文
| 作者 | Wang, Yuwei1,3; Suo, Yourui1,2; Sun, Yanan1,3; You, Jinmao1,2 |
| 刊名 | chromatographia
 |
| 出版日期 | 2016-11-01 |
| 卷号 | 79期号:21-22页码:1515-1525 |
| 关键词 | Ultrasound-assisted dispersive liquid-liquid microextraction (UA-DLLME) Pre-column derivatization High-performance liquid chromatography (HPLC) Benzimidazo-[2,1-b] quinazolin-12(6H)-one-5-ethyl-p-toluenesulfonate (BQETS) Raspberry Triterpene acids |
| 英文摘要 | in this work, we have developed an efficient method for the rapid extraction and separation of triterpene acids from 37 different varieties of raspberry via ultrasound-assisted dispersive liquid-liquid microextraction (ua-dllme). the triterpene acids were then determined by high-performance liquid chromatography (hplc) with fluorescence detection using benzimidazo-[2,1-b]quinazolin-12(6h)-one-5-ethyl-p-toluenesulfonate (bqets) as the labeling agent. five triterpene acids, including asiatic acid (aa), maslinic acid (ma), corosolic acid (ca), oleanolic acid (oa) and betulinic acid (ba), were extracted by ua-dllme using chloroform and acetone as the extracting and dispersing solvents, respectively. after extraction and nitrogen flushing, the extracts were simultaneously characterized by hplc based on pre-column derivatization using bqets, a new labeling agent synthesized in our laboratory. several key parameters affecting the extraction efficiency and derivatization yields were investigated and optimized by response surface methodology (rsm) combined with box-behnken design (bbd). the method was further validated for linearity (correlation coefficient r (2) > 0.9979), precision (rsd = 0.23-2.45 %), and recovery (rsd = 90-106.5 %). the limits of detection (lods) and the limits of quantification (loqs) were determined to be within the range of 1.83-7.69 a mu g/l and 6.06-25.47 a mu g/l, respectively. this is the first report of the use of bqets as a pre-column derivatization agent for the determination of triterpene acids in real samples. the proposed method has been applied to the determination of five triterpene acids in 37 different raspberry varieties with significantly increased sensitivity compared to other methods. the results obtained indicate that the contents of triterpene acids vary significantly across different raspberry varieties. |
| WOS标题词 | science & technology ; life sciences & biomedicine ; physical sciences |
| 类目[WOS] | biochemical research methods ; chemistry, analytical |
| 研究领域[WOS] | biochemistry & molecular biology ; chemistry |
| 关键词[WOS] | liquid-liquid microextraction ; thin-layer-chromatography ; tandem mass-spectrometry ; oleanolic acid ; ursolic acid ; ms method ; antioxidant capacity ; red raspberries ; quantification ; ellagitannins |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000387326700009 |
| 源URL | [http://ir.nwipb.ac.cn/handle/363003/6670]  |
| 专题 | 西北高原生物研究所_中国科学院西北高原生物研究所 |
| 作者单位 | 1.Chinese Acad Sci, Northwest Inst Plateau Biol, Key Lab Tibetan Med Res, Xining, Peoples R China 2.Qinghai Univ, State Key Lab Plateau Ecol & Agr, Xining, Peoples R China 3.Univ Chinese Acad Sci, Beijing, Peoples R China |
| 推荐引用方式 GB/T 7714 | Wang, Yuwei,Suo, Yourui,Sun, Yanan,et al. Determination of Triterpene Acids from 37 Different Varieties of Raspberry using Pre-column Derivatization and HPLC Fluorescence Detection[J]. chromatographia,2016,79(21-22):1515-1525. |
| APA | Wang, Yuwei,Suo, Yourui,Sun, Yanan,&You, Jinmao.(2016).Determination of Triterpene Acids from 37 Different Varieties of Raspberry using Pre-column Derivatization and HPLC Fluorescence Detection.chromatographia,79(21-22),1515-1525. |
| MLA | Wang, Yuwei,et al."Determination of Triterpene Acids from 37 Different Varieties of Raspberry using Pre-column Derivatization and HPLC Fluorescence Detection".chromatographia 79.21-22(2016):1515-1525. |
入库方式： OAI收割
来源：西北高原生物研究所
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