Nano-layered magnesium fluoride reservoirs on biomaterial surfaces strengthen polymorphonuclear leukocyte resistance to bacterial pathogens
文献类型：期刊论文
| 作者 | Guo, Geyong1; Zhou, Huaijuan2; Wang, Qiaojie1; Wang, Jiaxing1; Tan, Jiaqi1; Li, Jinhua2,3; Jin, Ping2; Shen, Hao1 |
| 刊名 | NANOSCALE
 |
| 出版日期 | 2017 |
| 卷号 | 9期号:2页码:875-892 |
| 英文摘要 | Biomaterial-related bacterial infections cause patient suffering, mortality and extended periods of hospitalization, imposing a substantial burden on medical systems. In this context, understanding of nanomaterials-bacteria-cells interactions is of both fundamental and clinical significance. Herein, nano-MgF2 films were deposited on titanium substrate via magnetron sputtering. Using this platform, the antibacterial behavior and mechanism of the nano-MgF2 films were investigated in vitro and in vivo. It was found that, for S. aureus (CA-MRSA, USA300) and S. epidermidis (RP62A), the nano-MgF2 films possessed excellent anti-biofilm activity, but poor anti-planktonic bacteria activity in vitro. Nevertheless, both the traditional SD rat osteomyelitis model and the novel stably luminescent mouse infection model demonstrated that nano-MgF2 films exerted superior anti-infection effect in vivo, which cannot be completely explained by the antibacterial activity of the nanomaterial itself. Further, using polymorphonuclear leukocytes (PMNs), the critical immune cells of innate immunity, a complementary investigation of MgF2-bacteria-PMNs co-culturing revealed that the nano-MgF2 films improved the antibacterial effect of PMNs through enhancing their phagocytosis and stability. To our knowledge, this is the first time of exploring the antimicrobial mechanism of nano-MgF2 from the perspective of innate immunity both in vitro and in vivo. Based on the research results, a plausible mechanism is put forward for the predominant antibacterial effect of nano-MgF2 in vivo, which may originate from the indirect immune enhancement effect of nano-MgF2 films. In summary, this study of surface antibacterial design using MgF2 nanolayer is a meaningful attempt, which can promote the host innate immune response to bacterial pathogens. This may give us a new understanding towards the antibacterial behavior and mechanism of nano-MgF2 films and pave the way towards their clinical applications. |
| WOS标题词 | Science & Technology ; Physical Sciences ; Technology |
| 类目[WOS] | Chemistry, Multidisciplinary ; Nanoscience & Nanotechnology ; Materials Science, Multidisciplinary ; Physics, Applied |
| 研究领域[WOS] | Chemistry ; Science & Technology - Other Topics ; Materials Science ; Physics |
| 关键词[WOS] | ZN-ZR ALLOY ; STAPHYLOCOCCUS-AUREUS ; IN-VITRO ; REAL-TIME ; MECHANICAL CHARACTERIZATION ; ANTIBACTERIAL PROPERTIES ; VIRULENCE DETERMINANT ; ANTIBIOFILM ACTIVITY ; HUMAN NEUTROPHILS ; INFECTION MODEL |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000394780200048 |
| 源URL | [http://ir.sic.ac.cn/handle/331005/23569]  |
| 专题 | 上海硅酸盐研究所_古陶瓷与工业陶瓷工程研究中心_期刊论文 |
| 作者单位 | 1.Shanghai Jiao Tong Univ, Affiliated Peoples Hosp 6, Dept Orthopaed, Shanghai 200233, Peoples R China 2.Chinese Acad Sci, Shanghai Inst Ceram, State Key Lab High Performance Ceram & Superfine, Shanghai 200050, Peoples R China 3.Univ Chinese Acad Sci, Beijing 100049, Peoples R China |
| 推荐引用方式 GB/T 7714 | Guo, Geyong,Zhou, Huaijuan,Wang, Qiaojie,et al. Nano-layered magnesium fluoride reservoirs on biomaterial surfaces strengthen polymorphonuclear leukocyte resistance to bacterial pathogens[J]. NANOSCALE,2017,9(2):875-892. |
| APA | Guo, Geyong.,Zhou, Huaijuan.,Wang, Qiaojie.,Wang, Jiaxing.,Tan, Jiaqi.,...&Shen, Hao.(2017).Nano-layered magnesium fluoride reservoirs on biomaterial surfaces strengthen polymorphonuclear leukocyte resistance to bacterial pathogens.NANOSCALE,9(2),875-892. |
| MLA | Guo, Geyong,et al."Nano-layered magnesium fluoride reservoirs on biomaterial surfaces strengthen polymorphonuclear leukocyte resistance to bacterial pathogens".NANOSCALE 9.2(2017):875-892. |
入库方式： OAI收割
来源：上海硅酸盐研究所
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