Incorporation of cerium oxide into hydroxyapatite coating regulates osteogenic activity of mesenchymal stem cell and macrophage polarization
文献类型：期刊论文
| 作者 | Li, Kai; Shen, Qingyi; Xie, Youtao; You, Mingyu; Huang, Liping; Zheng, Xuebin1 |
| 刊名 | JOURNAL OF BIOMATERIALS APPLICATIONS
 |
| 出版日期 | 2017-02-01 |
| 卷号 | 31期号:7页码:1062-1076 |
| 关键词 | CeO2 hydroxyapatite plasma spray bone mesenchymal stem cell macrophage |
| 英文摘要 | Biomedical coatings for orthopedic implants should facilitate osseointegration and mitigate implant-induced inflammatory reactions. Cerium oxide (CeO2) ceramics possess anti-oxidative properties and can be used to decrease mediators of inflammation, which makes them attractive for biomedical applications. In our work, two kinds of CeO2 incorporated hydroxyapatite coatings (HA-10Ce and HA-30Ce) were prepared via plasma spraying technique and the effects of CeO2 addition on the responses of bone mesenchymal stem cells (BMSCs) and RAW264.7 macrophages were investigated. An increase in CeO2 content in the HA coatings resulted in better osteogenic behaviors of BMSCs in terms of cell proliferation, alkaline phosphatase (ALP) activity and mineralized nodule formation. RT-PCR and western blot analysis suggested that the incorporation of CeO2 may promote the osteogenic differentiation of BMSCs through the Smad-dependent BMP signaling pathway, which activated Runx2 expression and subsequently enhanced the expression of ALP and OCN. The expression profiles of macrophages cultured on the CeO2 modified coating revealed a tendency toward a M2 phenotype, because of an upregulation of M2 surface markers (CD163 and CD206), anti-inflammatory cytokines (TNF- and IL-6) and osteoblastogenesis-related genes (BMP2 and TGF-1) as well as a downregulation of M1 surface markers (CCR7 and CD11c), proinflammatory cytokines (IL-10 and IL-1ra) and reactive oxygen species production. The results suggested the regulation of BMSCs behaviors and macrophage-mediated responses at the coating's surface were associated with CeO2 incorporation. The incorporation of CeO2 in HA coatings can be a valuable strategy to promote osteogenic responses and reduce inflammatory reactions. |
| WOS标题词 | Science & Technology ; Technology |
| 类目[WOS] | Engineering, Biomedical ; Materials Science, Biomaterials |
| 研究领域[WOS] | Engineering ; Materials Science |
| 关键词[WOS] | H2O2-INDUCED OXIDATIVE STRESS ; SIGNALING PATHWAY ; OSTEOBLAST DIFFERENTIATION ; CALCIUM SILICATE ; NANOPARTICLES ; BIOCERAMICS ; PERFORMANCE ; STATE |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000394652300008 |
| 源URL | [http://ir.sic.ac.cn/handle/331005/23709]  |
| 专题 | 上海硅酸盐研究所_特种无机涂层重点实验室_期刊论文 |
| 作者单位 | 1.Chinese Acad Sci, Shanghai Inst Ceram, 1295 Dingxi Rd, Shanghai 200050, Peoples R China 2.Shanghai Stomatol Dis Ctr, Shanghai, Peoples R China |
| 推荐引用方式 GB/T 7714 | Li, Kai,Shen, Qingyi,Xie, Youtao,et al. Incorporation of cerium oxide into hydroxyapatite coating regulates osteogenic activity of mesenchymal stem cell and macrophage polarization[J]. JOURNAL OF BIOMATERIALS APPLICATIONS,2017,31(7):1062-1076. |
| APA | Li, Kai,Shen, Qingyi,Xie, Youtao,You, Mingyu,Huang, Liping,&Zheng, Xuebin.(2017).Incorporation of cerium oxide into hydroxyapatite coating regulates osteogenic activity of mesenchymal stem cell and macrophage polarization.JOURNAL OF BIOMATERIALS APPLICATIONS,31(7),1062-1076. |
| MLA | Li, Kai,et al."Incorporation of cerium oxide into hydroxyapatite coating regulates osteogenic activity of mesenchymal stem cell and macrophage polarization".JOURNAL OF BIOMATERIALS APPLICATIONS 31.7(2017):1062-1076. |
入库方式： OAI收割
来源：上海硅酸盐研究所
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