PR-PR: Cross-Platform Laboratory Automation System
文献类型：期刊论文
| 作者 | Linshiz, Gregory1,2,3; Stawski, Nina1,2; Goyal, Garima1,2; Bi, Changhao2,4,5; Poust, Sean6; Sharma, Monica2; Mutalik, Vivek2; Keasling, Jay D.1,2,6; Hillson, Nathan J.1,2,3 |
| 刊名 | ACS SYNTHETIC BIOLOGY
 |
| 出版日期 | 2014-08-01 |
| 卷号 | 3期号:8页码:515-524 |
| 关键词 | laboratory automation microfluidics liquid-handling robot standardization DNA assembly Kunkel DNA mutagenesis |
| 英文摘要 | To enable protocol standardization, sharing, and efficient implementation across laboratory automation platforms, we have further developed the PR-PR open-source high-level biology-friendly robot programming language as a cross-platform laboratory automation system. Beyond liquid-handling robotics, PR-PR now supports microfluidic and microscopy platforms, as well as protocol translation into human languages, such as English. While the same set of basic PR-PR commands and features are available for each supported platform, the underlying optimization and translation modules vary from platform to platform. Here, we describe these further developments to PR-PR, and demonstrate the experimental implementation and validation of PR-PR protocols for combinatorial modified Golden Gate DNA assembly across liquid-handling robotic, microfluidic, and manual platforms. To further test PR-PR cross-platform performance, we then implement and assess PR-PR protocols for Kunkel DNA mutagenesis and hierarchical Gibson DNA assembly for microfluidic and manual platforms. |
| WOS标题词 | Science & Technology ; Life Sciences & Biomedicine |
| 类目[WOS] | Biochemical Research Methods |
| 研究领域[WOS] | Biochemistry & Molecular Biology |
| 关键词[WOS] | ONE-STEP ; ONE-POT ; EXPRESSION |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000340517000002 |
| 源URL | [http://124.16.173.210/handle/834782/2267]  |
| 专题 | 天津工业生物技术研究所_代谢工程与合成生物技术研究组 毕昌昊_期刊论文 |
| 作者单位 | 1.Joint BioEnergy Inst, Fuels Synth Div, Emeryville, CA 94608 USA 2.Univ Calif Berkeley, Lawrence Berkeley Natl Lab, Phys Biosci Div, Berkeley, CA 94720 USA 3.DOE Joint Genome Inst, Walnut Creek, CA 94598 USA 4.Chinese Acad Sci, Tianjin Inst Ind Biotechnol, Tianjin, Peoples R China 5.Chinese Acad Sci, Key Lab Syst Microbial Biotechnol, Tianjin, Peoples R China 6.Univ Calif Berkeley, Dept Bioengn, Dept Chem & Biomol Engn, Berkeley, CA 94720 USA |
| 推荐引用方式 GB/T 7714 | Linshiz, Gregory,Stawski, Nina,Goyal, Garima,et al. PR-PR: Cross-Platform Laboratory Automation System[J]. ACS SYNTHETIC BIOLOGY,2014,3(8):515-524. |
| APA | Linshiz, Gregory.,Stawski, Nina.,Goyal, Garima.,Bi, Changhao.,Poust, Sean.,...&Hillson, Nathan J..(2014).PR-PR: Cross-Platform Laboratory Automation System.ACS SYNTHETIC BIOLOGY,3(8),515-524. |
| MLA | Linshiz, Gregory,et al."PR-PR: Cross-Platform Laboratory Automation System".ACS SYNTHETIC BIOLOGY 3.8(2014):515-524. |
入库方式： OAI收割
来源：天津工业生物技术研究所
浏览0
下载0
收藏0
其他版本
除非特别说明，本系统中所有内容都受版权保护，并保留所有权利。