Combinatory optimization of chromosomal integrated mevalonate pathway for beta-carotene production in Escherichia coli
文献类型:期刊论文
作者 | Ye, Lijun1,2; Zhang, Chunzhi1; Bi, Changhao2,3; Li, Qingyan2,3; Zhang, Xueli2,3 |
刊名 | MICROBIAL CELL FACTORIES |
出版日期 | 2016-12-01 |
卷号 | 15 |
关键词 | Escherichia coli Isoprenoid beta-carotene MVA pathway RBS |
英文摘要 | Background: Plasmid expression is a popular method in studies of MVA pathway for isoprenoid production in Escherichia coli. However, heterologous gene expression with plasmid is often not stable and might burden growth of host cells, decreases cell mass and product yield. In this study, MVA pathway was divided into three modules, and two heterologous modules were integrated into the E. coli chromosome. These modules were individually modulated with regulatory parts to optimize efficiency of the pathway in terms of downstream isoprenoid production. |
WOS标题词 | Science & Technology ; Life Sciences & Biomedicine |
类目[WOS] | Biotechnology & Applied Microbiology |
研究领域[WOS] | Biotechnology & Applied Microbiology |
关键词[WOS] | ISOPENTENYL DIPHOSPHATE ; LYCOPENE PRODUCTION ; SUCCINATE PRODUCTION ; ISOPRENOID PATHWAY ; EXPRESSION ; BIOSYNTHESIS ; TERPENOIDS ; MICROORGANISMS ; ASTAXANTHIN ; LIMONENE |
收录类别 | SCI |
语种 | 英语 |
WOS记录号 | WOS:000389006900001 |
源URL | [http://124.16.173.210/handle/834782/2289] |
专题 | 天津工业生物技术研究所_代谢工程与合成生物技术研究组 毕昌昊_期刊论文 |
作者单位 | 1.Dalian Polytech Univ, Sch Biol Engn, Dalian 116034, Peoples R China 2.Chinese Acad Sci, Tianjin Inst Ind Biotechnol, Tianjin 300308, Peoples R China 3.Chinese Acad Sci, Key Lab Syst Microbial Biotechnol, Tianjin 300308, Peoples R China |
推荐引用方式 GB/T 7714 | Ye, Lijun,Zhang, Chunzhi,Bi, Changhao,et al. Combinatory optimization of chromosomal integrated mevalonate pathway for beta-carotene production in Escherichia coli[J]. MICROBIAL CELL FACTORIES,2016,15. |
APA | Ye, Lijun,Zhang, Chunzhi,Bi, Changhao,Li, Qingyan,&Zhang, Xueli.(2016).Combinatory optimization of chromosomal integrated mevalonate pathway for beta-carotene production in Escherichia coli.MICROBIAL CELL FACTORIES,15. |
MLA | Ye, Lijun,et al."Combinatory optimization of chromosomal integrated mevalonate pathway for beta-carotene production in Escherichia coli".MICROBIAL CELL FACTORIES 15(2016). |
入库方式: OAI收割
来源:天津工业生物技术研究所
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