Multivalent S-sialoside protein conjugates block influenza hemagglutinin and neuraminidase
文献类型：期刊论文
| 作者 | Yang, Yang1; Liu, Hai-Peng1; Yu, Qun1; Yang, Mei-Bing1; Wang, De-Min1; Jia, Tian-Wei1; He, Hao-Jie1; He, Yun2; Xiao, Hai-Xia3; Iyer, Suri S.4 |
| 刊名 | CARBOHYDRATE RESEARCH
 |
| 出版日期 | 2016-11-29 |
| 卷号 | 435页码:68-75 |
| 关键词 | Multivalent effect Glycoprotein Click chemistry Antiviral inhibitor |
| 英文摘要 | A new class of S-sialoside Human Serum Albumin (HSA) and Bovine Serum Albumin (BSA) conjugates were prepared to enhance the binding affinity to hemagglutinin (HA) and neuraminidase (NA). The valency of glycoconjugates was controlled by the reaction ratio of the S-sialoside monomer and protein. Hemagglutination inhibition assay showed that these synthetic glycoproteins have higher affinity to HA than the small clusters of sialosides with lower valency, due to multivalent effect and optimized three dimensional presentation of sialosides on the protein platform. The results of fluorescent NA inhibition assay showed that some of the conjugates have moderate NA inhibitory activity, in comparison to the monomer and low valent conjugates with weak or none inhibitory activity. These synthetic sialylated proteins were not cytotoxic with concentrations up to 100 mu M, since the sialylation did not change the secondary structure of protein. This new kind of conjugates can be used as lead compounds for antiviral drug design and the construction of pseudo sialoside-protein conjugates library to investigate the carbohydrate-HA/NA recognition process and a platform for the influenza virus capturing. (C) 2016 Elsevier Ltd. All rights reserved. |
| WOS标题词 | Science & Technology ; Life Sciences & Biomedicine ; Physical Sciences |
| 类目[WOS] | Biochemistry & Molecular Biology ; Chemistry, Applied ; Chemistry, Organic |
| 研究领域[WOS] | Biochemistry & Molecular Biology ; Chemistry |
| 关键词[WOS] | SIALIC-ACID ; CHEMOENZYMATIC SYNTHESIS ; PHOSPHONATE CONGENERS ; VIRUS HEMAGGLUTININ ; ANTIVIRAL AGENTS ; LECTIN-BINDING ; A VIRUS ; INHIBITORS ; INFECTION ; NANOPARTICLES |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000389399200008 |
| 源URL | [http://124.16.173.210/handle/834782/2285]  |
| 专题 | 天津工业生物技术研究所_蛋白质工程与疫苗实验室 高福_期刊论文 |
| 作者单位 | 1.Tianjin Univ Sci & Technol, China Int Sci & Technol Cooperat Base Food Nutr, Coll Biotechnol,Sino French Joint Lab Food Nutr S, Key Lab Ind Fermentat Microbiol,Minist Educ,Tianj, Tianjin 300457, Peoples R China 2.Atlanta Res & Educ Fdn, Atlanta, GA 30329 USA 3.Chinese Acad Sci, Tianjin Inst Ind Biotechnol, Tianjin 300308, Peoples R China 4.Georgia State Univ, Dept Chem, Ctr Diagnost & Therapeut, Atlanta, GA 30302 USA 5.Tianjin Univ Sci & Technol, Coll Food Engn & Biotechnol, Minist Educ, Key Lab Food Nutr & Safety, Tianjin 300457, Peoples R China |
| 推荐引用方式 GB/T 7714 | Yang, Yang,Liu, Hai-Peng,Yu, Qun,et al. Multivalent S-sialoside protein conjugates block influenza hemagglutinin and neuraminidase[J]. CARBOHYDRATE RESEARCH,2016,435:68-75. |
| APA | Yang, Yang.,Liu, Hai-Peng.,Yu, Qun.,Yang, Mei-Bing.,Wang, De-Min.,...&Yu, Peng.(2016).Multivalent S-sialoside protein conjugates block influenza hemagglutinin and neuraminidase.CARBOHYDRATE RESEARCH,435,68-75. |
| MLA | Yang, Yang,et al."Multivalent S-sialoside protein conjugates block influenza hemagglutinin and neuraminidase".CARBOHYDRATE RESEARCH 435(2016):68-75. |
入库方式： OAI收割
来源：天津工业生物技术研究所
浏览0
下载0
收藏0
其他版本
除非特别说明，本系统中所有内容都受版权保护，并保留所有权利。