Intra-host dynamics of Ebola virus during 2014
文献类型：期刊论文
| 作者 | Ni, Ming1; Chen, Chen2,3; Qian, Jun4; Xiao, Hai-Xia5,6; Shi, Wei-Feng7; Luo, Yang8; Wang, Hai-Yin2; Li, Zhen1; Wu, Jun5; Xu, Pei-Song9 |
| 刊名 | Nature Microbiology
 |
| 出版日期 | 2016-11-01 |
| 卷号 | 1期号:11 |
| 英文摘要 | Since 2013, West Africa has encountered the largest Ebola virus (EBOV) disease outbreak on record, and Sierra Leone is the worst-affected country, with nearly half of the infections. By means of next-generation sequencing and phylogeographic analysis, the epidemiology and transmission of EBOV have been well elucidated. However, the intra-host dynamics that mainly reflect viral-host interactions still need to be studied. Here, we show a total of 710 intra-host single nucleotide variations (iSNVs) from deep-sequenced samples from EBOV-infected patients, through a well-tailored bioinformatics pipeline. We present a comprehensive distribution of iSNVs during this outbreak and along the EBOV genome. Analyses of iSNV and its allele frequency reveal that VP40 is the most conserved gene during this outbreak, and thus it would be an ideal therapeutic target. In the co-occurring iSNV network, varied iSNV sites present different selection features. Intriguingly, the T-to-C substitutions at the 3'-UTR of the nucleoprotein (NP; positions 3008 and 3011), observed in many patients, result in the upregulation of the transcription of NP through an Ebola mini-genome reporting system. Additionally, no iSNV enrichment within B-cell epitopes of GP has been observed. |
| WOS标题词 | Science & Technology ; Life Sciences & Biomedicine |
| 类目[WOS] | Microbiology |
| 研究领域[WOS] | Microbiology |
| 关键词[WOS] | SIERRA-LEONE ; PROTEIN ; TRANSMISSION ; DIVERSITY ; EVOLUTION ; ALIGNMENT ; OUTBREAK ; AFRICA ; HIV-1 ; VP40 |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000389139500007 |
| 源URL | [http://124.16.173.210/handle/834782/2287]  |
| 专题 | 天津工业生物技术研究所_蛋白质工程与疫苗实验室 高福_期刊论文 |
| 作者单位 | 1.Beijing Inst Radiat Med, Beijing 100850, Peoples R China 2.Capital Med Univ, Beijing Ditan Hosp, Inst Infect Dis, Beijing 100102, Peoples R China 3.Beijing Key Lab Emerging Infect Dis, Beijing 100102, Peoples R China 4.Key Lab Jilin Prov Zoonosis Prevent & Control, Changchun 130122, Peoples R China 5.Chinese Acad Sci, Inst Microbiol, CAS Key Lab Pathogen Microbiol & Immunol, Beijing 100101, Peoples R China 6.Chinese Acad Sci, Tianjin Inst Ind Biotechnol, Lab Prot Engn & Vaccines, Tianjin 300308, Peoples R China 7.Inst Pathogen Biol, Taishan Med Coll, Tai An 271016, Shandong, Peoples R China 8.Wellcome Trust Sanger Inst, Cambridge CB10 1SA, England 9.Zhiyuan Med Inspect Inst, Guangzhou 310009, Zhejiang, Peoples R China 10.Shenzhen Third Peoples Hosp, Shenzhen Key Lab Pathogen & Immun, Shenzhen 518112, Peoples R China |
| 推荐引用方式 GB/T 7714 | Ni, Ming,Chen, Chen,Qian, Jun,et al. Intra-host dynamics of Ebola virus during 2014[J]. Nature Microbiology,2016,1(11). |
| APA | Ni, Ming.,Chen, Chen.,Qian, Jun.,Xiao, Hai-Xia.,Shi, Wei-Feng.,...&Liu, Di.(2016).Intra-host dynamics of Ebola virus during 2014.Nature Microbiology,1(11). |
| MLA | Ni, Ming,et al."Intra-host dynamics of Ebola virus during 2014".Nature Microbiology 1.11(2016). |
入库方式： OAI收割
来源：天津工业生物技术研究所
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