The NS1 gene from bat-derived influenza-like virus H17N10 can be rescued in influenza A PR8 backbone
文献类型：期刊论文
| 作者 | Zhao, Xuejin1,2,3; Tefsen, Boris1,3,4; Li, Yan1,3; Qi, Jianxun1,3; Lu, Guangwen1,3; Shi, Yi3,5; Yan, Jinghua1,2; Xiao, Haixia3,6; Gao, George F.1,3,5,6,7 |
| 刊名 | JOURNAL OF GENERAL VIROLOGY
 |
| 出版日期 | 2016-08-01 |
| 卷号 | 97页码:1797-1806 |
| 英文摘要 | Influenza A viruses have the potential to cause pandemics due to the introduction of novel subtypes against which human hosts have little or no preexisting immunity. Such viruses may result from reassortment between human and animal influenza viruses. Recently, new influenza-like viruses were identified in bats, raising the concern for a new reservoir of potentially harmful influenza viruses that could form reassortants with categorized human influenza A viruses. However, until now, it has not been possible to generate a recombinant reassortant virus containing a single functional gene or domain from H17N10 that could propagate. Here, we demonstrate that a recombinant A/Puerto Rico/8/1934 (H1N1) virus with NS1 gene from H17N10 influenza-like virus can be successfully rescued. We used luciferase reporter assays and quantitative reverse transcriptase PCR to show that the NS1 protein from H17N10 inhibited Sendai-virus (SeV)-induced activation of IFN-beta expression with an efficiency similar to NS1 from an H5N1 strain. Moreover, the crystal structure of the NS1 ( H17N10) RNA-binding domain is also similar to that of other NS1s. These results demonstrate that H17N10 influenza-like virus indeed contains functional genes that are compatible with categorized influenza A viruses. Although the chance of this particular event occurring in nature seems negligible, further research is needed to address the possibility of the natural formation of reassortants. |
| WOS标题词 | Science & Technology ; Life Sciences & Biomedicine |
| 类目[WOS] | Biotechnology & Applied Microbiology ; Virology |
| 研究领域[WOS] | Biotechnology & Applied Microbiology ; Virology |
| 关键词[WOS] | DOUBLE-STRANDED-RNA ; X-RAY-STRUCTURE ; NUCLEAR EXPORT ; CRYSTAL-STRUCTURE ; RECEPTOR-BINDING ; EFFECTOR DOMAIN ; HUMAN INFECTION ; H7N9 VIRUS ; 5 ENDS ; PROTEIN |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000385288500007 |
| 源URL | [http://124.16.173.210/handle/834782/2941]  |
| 专题 | 天津工业生物技术研究所_蛋白质工程与疫苗实验室 高福_期刊论文 |
| 作者单位 | 1.Chinese Acad Sci, Inst Microbiol, CAS Key Lab Pathogen Microbiol & Immunol, Beijing 100101, Peoples R China 2.Chinese Acad Sci, Inst Microbiol, CAS Key Lab Microbial Physiol & Metab Engn, Beijing 100101, Peoples R China 3.Chinese Acad Sci, Ctr Influenza Res & Early Warning CASCIRE, Beijing 100101, Peoples R China 4.Xian Jiaotong Liverpool Univ, Dept Biol Sci, Dushu Lake Higher Educ Town, 111 Ren Ai Rd,Suzhou Ind Pk, Suzhou 215123, Peoples R China 5.Chinese Acad Sci, Beijing Inst Life Sci, Beijing 100101, Peoples R China 6.Chinese Acad Sci, Tianjin Inst Ind Biotechnol, Tianjin 300308, Peoples R China 7.Chinese Ctr Dis Control & Prevent, Off Director Gen, Beijing 102206, Peoples R China |
| 推荐引用方式 GB/T 7714 | Zhao, Xuejin,Tefsen, Boris,Li, Yan,et al. The NS1 gene from bat-derived influenza-like virus H17N10 can be rescued in influenza A PR8 backbone[J]. JOURNAL OF GENERAL VIROLOGY,2016,97:1797-1806. |
| APA | Zhao, Xuejin.,Tefsen, Boris.,Li, Yan.,Qi, Jianxun.,Lu, Guangwen.,...&Gao, George F..(2016).The NS1 gene from bat-derived influenza-like virus H17N10 can be rescued in influenza A PR8 backbone.JOURNAL OF GENERAL VIROLOGY,97,1797-1806. |
| MLA | Zhao, Xuejin,et al."The NS1 gene from bat-derived influenza-like virus H17N10 can be rescued in influenza A PR8 backbone".JOURNAL OF GENERAL VIROLOGY 97(2016):1797-1806. |
入库方式： OAI收割
来源：天津工业生物技术研究所
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