Pathway Construction in Corynebacterium glutamicum and Strain Engineering To Produce Rare Sugars from Glycerol
文献类型：期刊论文
| 作者 | Yang, Jiangang; Zhu, Yueming; Men, Yan; Sun, Shangshang; Zeng, Yan; Zhang, Ying; Sun, Yuanxia; Ma, Yanhe |
| 刊名 | JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
 |
| 出版日期 | 2016-12-21 |
| 卷号 | 64期号:50页码:9497-9505 |
| 关键词 | rare sugars aldolases Corynebacterium glutamicum metabolic engineering glycerol |
| 英文摘要 | Rare sugars are valuable natural products widely used in pharmaceutical and food industries. In this study, we expected to synthesize rare ketoses from abundant glycerol using dihydroxyacetone phosphate (DHAP)-dependent aldolases. First, a new glycerol assimilation pathway was constructed to synthesize DHAP. The enzymes which convert glycerol to 3-hydroxypropionaldehyde and L-glyceraldehyde were selected, and their corresponding aldehyde synthesis pathways were constructed in vivo. Four aldol pathways based on different aldolases and phosphorylase were gathered. Next, three pathways were assembled and the resulting strains synthesized 5-deoxypsicose, 5-deoxysorbose, and 5-deoxyfructose from glucose and glycerol and produce L-fructose, L-tagatose, L-sorbose, and L-psicose with glycerol as the only carbon source. To achieve higher product titer and yield, the recombinant strains were further engineered and fermentation conditions were optimized. Fed-batch culture of engineered strains obtained 38.1 g/L 5-deoxypsicose with a yield of 0.91 +/- 0.04 mol product per mol of glycerol and synthesized 20.8 g/L L-fructose, 10.3 g/L L-tagatose, 1.2 g/L L-sorbose, and 0.95 g/L L-psicose. |
| WOS标题词 | Science & Technology ; Life Sciences & Biomedicine ; Physical Sciences |
| 类目[WOS] | Agriculture, Multidisciplinary ; Chemistry, Applied ; Food Science & Technology |
| 研究领域[WOS] | Agriculture ; Chemistry ; Food Science & Technology |
| 关键词[WOS] | ESCHERICHIA-COLI ; SACCHAROMYCES-CEREVISIAE ; ACID PRODUCTION ; L-FRUCTOSE ; L-PSICOSE ; L-SORBOSE ; BIOSYNTHESIS ; DEHYDROGENASE ; ISOMERASE ; OVEREXPRESSION |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000390729100004 |
| 源URL | [http://124.16.173.210/handle/834782/2280]  |
| 专题 | 天津工业生物技术研究所_功能糖与天然活性物质研究组 孙媛霞 _期刊论文 |
| 作者单位 | Chinese Acad Sci, Tianjin Inst Ind Biotechnol, Natl Engn Lab Ind Enzymes, Tianjin 300308, Peoples R China |
| 推荐引用方式 GB/T 7714 | Yang, Jiangang,Zhu, Yueming,Men, Yan,et al. Pathway Construction in Corynebacterium glutamicum and Strain Engineering To Produce Rare Sugars from Glycerol[J]. JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY,2016,64(50):9497-9505. |
| APA | Yang, Jiangang.,Zhu, Yueming.,Men, Yan.,Sun, Shangshang.,Zeng, Yan.,...&Ma, Yanhe.(2016).Pathway Construction in Corynebacterium glutamicum and Strain Engineering To Produce Rare Sugars from Glycerol.JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY,64(50),9497-9505. |
| MLA | Yang, Jiangang,et al."Pathway Construction in Corynebacterium glutamicum and Strain Engineering To Produce Rare Sugars from Glycerol".JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY 64.50(2016):9497-9505. |
入库方式： OAI收割
来源：天津工业生物技术研究所
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