Complete genome sequence and transcriptomic analysis of a novel marine strain Bacillus weihaiensis reveals the mechanism of brown algae degradation
文献类型：期刊论文
| 作者 | Zhu, Yueming; Chen, Peng; Bao, Yunjuan; Men, Yan; Zeng, Yan; Yang, Jiangang; Sun, Jibin; Sun, Yuanxia |
| 刊名 | SCIENTIFIC REPORTS
 |
| 出版日期 | 2016-11-30 |
| 卷号 | 6 |
| 英文摘要 | A novel marine strain representing efficient degradation ability toward brown algae was isolated, identified, and assigned to Bacillus weihaiensis Alg07. The alga-associated marine bacteria promote the nutrient cycle and perform important functions in the marine ecosystem. The de novo sequencing of the B. weihaiensis Alg07 genome was carried out. Results of gene annotation and carbohydrate-active enzyme analysis showed that the strain harbored enzymes that can completely degrade alginate and laminarin, which are the specific polysaccharides of brown algae. We also found genes for the utilization of mannitol, the major storage monosaccharide in the cell of brown algae. To understand the process of brown algae decomposition by B. weihaiensis Alg07, RNA-seq transcriptome analysis and qRT-PCR were performed. The genes involved in alginate metabolism were all up-regulated in the initial stage of kelp degradation, suggesting that the strain Alg07 first degrades alginate to destruct the cell wall so that the laminarin and mannitol are released and subsequently decomposed. The key genes involved in alginate and laminarin degradation were expressed in Escherichia coli and characterized. Overall, the model of brown algae degradation by the marine strain Alg07 was established, and novel alginate lyases and laminarinase were discovered. |
| WOS标题词 | Science & Technology |
| 类目[WOS] | Multidisciplinary Sciences |
| 研究领域[WOS] | Science & Technology - Other Topics |
| 关键词[WOS] | SPHINGOMONAS SP A1 ; ALGINATE LYASE ; SUBSTRATE RECOGNITION ; ESCHERICHIA-COLI ; MAJOR SOURCES ; LAMINARIN ; BACTERIA ; DATABASE ; IDENTIFICATION ; PURIFICATION |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000389471200001 |
| 源URL | [http://124.16.173.210/handle/834782/2284]  |
| 专题 | 天津工业生物技术研究所_功能糖与天然活性物质研究组 孙媛霞 _期刊论文 |
| 作者单位 | Chinese Acad Sci, Tianjin Inst Ind Biotechnol, Natl Engn Lab Ind Enzymes, Tianjin 300308, Peoples R China |
| 推荐引用方式 GB/T 7714 | Zhu, Yueming,Chen, Peng,Bao, Yunjuan,et al. Complete genome sequence and transcriptomic analysis of a novel marine strain Bacillus weihaiensis reveals the mechanism of brown algae degradation[J]. SCIENTIFIC REPORTS,2016,6. |
| APA | Zhu, Yueming.,Chen, Peng.,Bao, Yunjuan.,Men, Yan.,Zeng, Yan.,...&Sun, Yuanxia.(2016).Complete genome sequence and transcriptomic analysis of a novel marine strain Bacillus weihaiensis reveals the mechanism of brown algae degradation.SCIENTIFIC REPORTS,6. |
| MLA | Zhu, Yueming,et al."Complete genome sequence and transcriptomic analysis of a novel marine strain Bacillus weihaiensis reveals the mechanism of brown algae degradation".SCIENTIFIC REPORTS 6(2016). |
入库方式： OAI收割
来源：天津工业生物技术研究所
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