High-level intra- and extra-cellular production of D-psicose 3-epimerase via a modified xylose-inducible expression system in Bacillus subtilis
文献类型：期刊论文
| 作者 | Chen, Jingqi1,2; Zhu, Yueming1,3; Fu, Gang1,3; Song, Yafeng1,2; Jin, Zhaoxia4; Sun, Yuanxia1,3; Zhang, Dawei1,2,3 |
| 刊名 | JOURNAL OF INDUSTRIAL MICROBIOLOGY & BIOTECHNOLOGY
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| 出版日期 | 2016-11-01 |
| 卷号 | 43期号:11页码:1577-1591 |
| 关键词 | D-Psicose 3-epimerase Xylose-inducible expression system xylAB Bacillus subtilis Protein secretion |
| 英文摘要 | d-Psicose 3-epimerase (DPEase) converts d-fructose into d-psicose which exists in nature in limited quantities and has key physiological functions. In this study, RDPE (DPEase from Ruminococcus sp. 5_1_39BFAA) was successfully constitutively expressed in Bacillus subtilis, which is the first report of its kind. Three sugar-inducible promoters were compared, and the xylose-inducible promoter P (xylA) was proved to be the most efficient for RDPE production. Based on the analysis of the inducer concentration and RDPE expression, we surmised that there was an extremely close correlation between the intracellular RDPE expression and xylose accumulation level. Subsequently, after the metabolic pathway of xylose was blocked by deletion of xylAB, the intra- and extra-cellular RDPE expression was significantly enhanced. Meanwhile, the optimal xylose induction concentration was reduced from 4.0 to 0.5 %. Eventually, the secretion level of RDPE reached 95 U/mL and 2.6 g/L in a 7.5-L fermentor with the fed-batch fermentation, which is the highest production of DPEase by a microbe to date. |
| WOS标题词 | Science & Technology ; Life Sciences & Biomedicine |
| 类目[WOS] | Biotechnology & Applied Microbiology |
| 研究领域[WOS] | Biotechnology & Applied Microbiology |
| 关键词[WOS] | COMPLETE GENOME SEQUENCE ; ESCHERICHIA-COLI ; PROTEIN SECRETION ; PSEUDOMONAS SP ; D-FRUCTOSE ; PROMOTER ; OVEREXPRESSION ; TRANSFORMATION ; KETOHEXOSE ; MEGATERIUM |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000386370600008 |
| 源URL | [http://124.16.173.210/handle/834782/2942]  |
| 专题 | 天津工业生物技术研究所_功能糖与天然活性物质研究组 孙媛霞 _期刊论文 |
| 作者单位 | 1.Chinese Acad Sci, Tianjin Inst Ind Biotechnol, Tianjin 300308, Peoples R China 2.Chinese Acad Sci, Key Lab Syst Microbial Biotechnol, Tianjin 300308, Peoples R China 3.Natl Engn Lab Ind Enzymes, Tianjin 300308, Peoples R China 4.Dalian Polytech Univ, Sch Biol Engn, Dalian 116034, Peoples R China |
| 推荐引用方式 GB/T 7714 | Chen, Jingqi,Zhu, Yueming,Fu, Gang,et al. High-level intra- and extra-cellular production of D-psicose 3-epimerase via a modified xylose-inducible expression system in Bacillus subtilis[J]. JOURNAL OF INDUSTRIAL MICROBIOLOGY & BIOTECHNOLOGY,2016,43(11):1577-1591. |
| APA | Chen, Jingqi.,Zhu, Yueming.,Fu, Gang.,Song, Yafeng.,Jin, Zhaoxia.,...&Zhang, Dawei.(2016).High-level intra- and extra-cellular production of D-psicose 3-epimerase via a modified xylose-inducible expression system in Bacillus subtilis.JOURNAL OF INDUSTRIAL MICROBIOLOGY & BIOTECHNOLOGY,43(11),1577-1591. |
| MLA | Chen, Jingqi,et al."High-level intra- and extra-cellular production of D-psicose 3-epimerase via a modified xylose-inducible expression system in Bacillus subtilis".JOURNAL OF INDUSTRIAL MICROBIOLOGY & BIOTECHNOLOGY 43.11(2016):1577-1591. |
入库方式： OAI收割
来源：天津工业生物技术研究所
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