CAH1 and CAH2 as key enzymes required for high bicarbonate tolerance of a novel microalga Dunaliella salina HTBS
文献类型：期刊论文
| 作者 | Hou, Yuyong1; Liu, Zhiyong1; Zhao, Yue1; Chen, Shulin1; Zheng, Yubin2; Chen, Fangjian1 |
| 刊名 | ENZYME AND MICROBIAL TECHNOLOGY
 |
| 出版日期 | 2016-06-01 |
| 卷号 | 87-88页码:17-23 |
| 关键词 | Dunaliella salina HTBS Inorganic carbon fixation Bicarbonate Carbonic anhydrase |
| 英文摘要 | Outdoor microalgal cultivation with high concentration bicarbonate has been considered as a strategy for reducing contamination and improving carbon supply efficiency. The mechanism responsible for algae's strong tolerance to high bicarbonate however, remains not clear. In this study, we isolated and characterized a strain and revealed its high bicarbonate tolerant mechanism by analyzing carbonic anhydrase (CA). The strain was identified as Dunaliella salina HTBS with broad temperature adaptability (7-30 degrees C). The strain grew well under 30% CO2 or 70 g L-1 NaHCO3. In comparison, two periplasm CAs (CAH1 and CAH2) were detected with immunoblotting analysis in HTBS but not in a non-HCO3--tolerant strain. The finding was also verified by an enzyme inhibition assay in which only HTBS showed significant inhibition by extracellular CA inhibitor. Thus, we inferred that the extracellular CAH1 and CAH2 played a multifunctional role in the toleration of high bicarbonate by HTBS. (C) 2016 Elsevier Inc. All rights reserved. |
| WOS标题词 | Science & Technology ; Life Sciences & Biomedicine |
| 类目[WOS] | Biotechnology & Applied Microbiology |
| 研究领域[WOS] | Biotechnology & Applied Microbiology |
| 关键词[WOS] | CARBONIC-ANHYDRASE ; CHLAMYDOMONAS-REINHARDTII ; CONCENTRATING MECHANISM ; MARINE DIATOM ; CO2 ; EXPRESSION ; DIOXIDE ; CAPTURE ; ASTAXANTHIN ; BIOFIXATION |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000376804100003 |
| 源URL | [http://124.16.173.210/handle/834782/2250]  |
| 专题 | 天津工业生物技术研究所_工业生物系统工程实验室 陈树林_期刊论文 |
| 作者单位 | 1.Chinese Acad Sci, Tianjin Inst Ind Biotechnol, Tianjin Key Lab Ind Biol Syst & Bioproc Engn, Tianjin 300308, Peoples R China 2.Shandong Jinjing Biotechnol Co Ltd, Boshan 261313, Shandong, Peoples R China |
| 推荐引用方式 GB/T 7714 | Hou, Yuyong,Liu, Zhiyong,Zhao, Yue,et al. CAH1 and CAH2 as key enzymes required for high bicarbonate tolerance of a novel microalga Dunaliella salina HTBS[J]. ENZYME AND MICROBIAL TECHNOLOGY,2016,87-88:17-23. |
| APA | Hou, Yuyong,Liu, Zhiyong,Zhao, Yue,Chen, Shulin,Zheng, Yubin,&Chen, Fangjian.(2016).CAH1 and CAH2 as key enzymes required for high bicarbonate tolerance of a novel microalga Dunaliella salina HTBS.ENZYME AND MICROBIAL TECHNOLOGY,87-88,17-23. |
| MLA | Hou, Yuyong,et al."CAH1 and CAH2 as key enzymes required for high bicarbonate tolerance of a novel microalga Dunaliella salina HTBS".ENZYME AND MICROBIAL TECHNOLOGY 87-88(2016):17-23. |
入库方式： OAI收割
来源：天津工业生物技术研究所
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