Genetic Incorporation of N-epsilon-Formyllysine, a New Histone Post-translational Modification
文献类型：期刊论文
| 作者 | Wang, Tianyuan1; Zhou, Qing2; Li, Fahui2; Yu, Yang3; Yin, Xuebin1,4; Wang, Jiangyun2 |
| 刊名 | CHEMBIOCHEM
 |
| 出版日期 | 2015-07-06 |
| 卷号 | 16期号:10页码:1440-1442 |
| 关键词 | amino acids formyllysine gene technology histones post-translational modifications |
| 英文摘要 | Lysine formylation is a newly discovered post-translational modification (PTM) in histones and other nuclear proteins; it has a well-recognized but poorly defined role in chromatin conformation modulation and gene expression. To date, there is no general method to site-specifically incorporate N-epsilon-formyllysine at a defined site of a protein. Here we report the highly efficient genetic incorporation of the unnatural amino acid N-epsilon-formyllysine into proteins produced in Escherichia coli and mammalian cells, by using an orthogonal N-epsilon-formyllysine tRNAsynthetase/tRNA(CUA) pair. This technique can be applied to study the role of lysine formylation in epigenetic regulation. |
| WOS标题词 | Science & Technology ; Life Sciences & Biomedicine |
| 类目[WOS] | Biochemistry & Molecular Biology ; Chemistry, Medicinal |
| 研究领域[WOS] | Biochemistry & Molecular Biology ; Pharmacology & Pharmacy |
| 关键词[WOS] | MAMMALIAN-CELLS ; PHOTOCLICK-CHEMISTRY ; HUMAN-DISEASE ; PROTEINS ; ACETYLATION ; LYSINE ; FORMYLATION ; CODE |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000357326200008 |
| 源URL | [http://124.16.173.210/handle/834782/2262]  |
| 专题 | 天津工业生物技术研究所_化学生物学研究 陆艺_期刊论文 |
| 作者单位 | 1.Univ Sci & Technol China, Sch Earth & Space Sci, Hefei 230026, Anhui, Peoples R China 2.Chinese Acad Sci, Inst Biophys, Lab RNA Biol, Beijing 100101, Peoples R China 3.Chinese Acad Sci, Inst Ind Biotechnol, Tianjin 300308, Peoples R China 4.USTC, Suzhou Inst Adv Study, Suzhou Key Lab Selenium & Human Hlth, Dushu Lake Higher Educ Area, Suzhou 215123, Jiangsu, Peoples R China |
| 推荐引用方式 GB/T 7714 | Wang, Tianyuan,Zhou, Qing,Li, Fahui,et al. Genetic Incorporation of N-epsilon-Formyllysine, a New Histone Post-translational Modification[J]. CHEMBIOCHEM,2015,16(10):1440-1442. |
| APA | Wang, Tianyuan,Zhou, Qing,Li, Fahui,Yu, Yang,Yin, Xuebin,&Wang, Jiangyun.(2015).Genetic Incorporation of N-epsilon-Formyllysine, a New Histone Post-translational Modification.CHEMBIOCHEM,16(10),1440-1442. |
| MLA | Wang, Tianyuan,et al."Genetic Incorporation of N-epsilon-Formyllysine, a New Histone Post-translational Modification".CHEMBIOCHEM 16.10(2015):1440-1442. |
入库方式： OAI收割
来源：天津工业生物技术研究所
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