Significant Enhancement of hPrx1 Chaperone Activity through Lysine Acetylation
文献类型：期刊论文
| 作者 | Pan, Yanchao1; Jin, Jing-Hua3; Yu, Yang2; Wang, Jiangyun1 |
| 刊名 | CHEMBIOCHEM
 |
| 出版日期 | 2014-08-18 |
| 卷号 | 15期号:12页码:1773-1776 |
| 关键词 | amino acids chaperone proteins genetic code expansion lysine acetylation post-translational modifications |
| 英文摘要 | The reversible acetylation of proteins plays a key role in regulating biological processes, including chromatin remodeling, progression of the cell cycle, and actin nucleation. Human peroxiredoxin 1(hPrx1), one of the most abundant proteins in the cytoplasm, has been shown to be acetylated in human liver-carcinoma tissues. However, little is known about what function(s) the acetylation serves for hPrx1. Herein, using the method of genetic code expansion, we incorporated Ne-acetyllysine (AcK) site-specifically into hPrx1. Our data showed that acetylation the K-27 residue promotes oligomerization of hPrx1 at low concentrations. In addition, K-27-acetylated hPrx1(hPrx1-AcK27) exhibited greatly enhanced chaperone activity (e. g. protecting the protein malate dehydrogenase (MDH) from thermally induced aggregation and assisting the refolding of denatured citrate synthase (CS)). These findings suggest that the site-specific acetylation of hPrx1 may change its biological role in response to environmental changes. |
| WOS标题词 | Science & Technology ; Life Sciences & Biomedicine |
| 类目[WOS] | Biochemistry & Molecular Biology ; Chemistry, Medicinal |
| 研究领域[WOS] | Biochemistry & Molecular Biology ; Pharmacology & Pharmacy |
| 关键词[WOS] | 2-CYS PEROXIREDOXIN ; PROTEINS ; PHOSPHORYLATION ; LOCALIZATION ; PEROXIDASE ; HDAC6 |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000340506200013 |
| 源URL | [http://124.16.173.210/handle/834782/2270]  |
| 专题 | 天津工业生物技术研究所_化学生物学研究 陆艺_期刊论文 |
| 作者单位 | 1.Chinese Acad Sci, Inst Biophys, Beijing 100101, Peoples R China 2.Chinese Acad Sci, Tianjin Inst Ind Biotechnol, Tianjin Airport Econ Area, Tianjin 300308, Peoples R China 3.Environm Protect Res Inst Light Ind, Beijing 100089, Peoples R China |
| 推荐引用方式 GB/T 7714 | Pan, Yanchao,Jin, Jing-Hua,Yu, Yang,et al. Significant Enhancement of hPrx1 Chaperone Activity through Lysine Acetylation[J]. CHEMBIOCHEM,2014,15(12):1773-1776. |
| APA | Pan, Yanchao,Jin, Jing-Hua,Yu, Yang,&Wang, Jiangyun.(2014).Significant Enhancement of hPrx1 Chaperone Activity through Lysine Acetylation.CHEMBIOCHEM,15(12),1773-1776. |
| MLA | Pan, Yanchao,et al."Significant Enhancement of hPrx1 Chaperone Activity through Lysine Acetylation".CHEMBIOCHEM 15.12(2014):1773-1776. |
入库方式： OAI收割
来源：天津工业生物技术研究所
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